Serum and colostrum/milk alkaline phosphatase activities in the determination of passive transfer status in healthy lambs
Yükleniyor...
Dosyalar
Tarih
2004
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ECOLE NATIONALE VETERINAIRE TOULOUSE
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to evaluate the importance of serum and colostrum/milk alkaline phosphatase (ALP) enzyme activity in the determination of passive transfer status in healthy lambs. Thirty Akkaraman Sheep (3-6 years old) which had normal pregnancy period and their 0 to 15 days old lambs (n=30) were used. Blood and colostrum/dmilk samples were collected from sheep and lambs after birth, before suckling (0 Day) and at the 1(st), 3(rd), 7(th) and 15(th) day. Serum IgG concentration was determined by the use of Single Radial Immunodiffusion (SRID) method. Serum ALP activity was measured, using a colorimetric kit. Correlations were carried out between immunoglobulin concentrations and ALP activities. Regression models (simple and multiple) were calculated. In lambs, although positive correlations were obtained between ALP activities and 1, G concentrations in serum at Days 1, 3 and 7 (r = 0.689, p < 0.01, r = 0.464, p < 0.05 and r = 0.413, p < 0.05 respectively), the variations of the 2 parameters showed marked discrepancies during the experiment : the ALP activity was maximum at day 0 whereas IgG concentrations were very low and from day 7 to day 15, ALP activity rose tip when IgG concentrations continued to decrease. From day 0 to day 7, ALP activities decreased in colostrum/milk whereas IgG concentrations were stable. Positive correlations between these 2 variables were only found on days 1 and 3 (r 0.404, p < 0.05 and r = 0.580, p < 0.01 respectively). Consequently, ALP activities were not strictly correlated with IgG concentrations nor in colostrum/milk either in lamb serum, and multiple regression models were not really suitable to calculate IgG concentrations. ALP activity is not Suitable for accurately predicting lamb IgG status and eventual failure for immune passive transfer.
Açıklama
Anahtar Kelimeler
IgG, ALP, lamb, milk/colostrum, serum
Kaynak
REVUE DE MEDECINE VETERINAIRE
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
155
Sayı
11
