Sığırlarda oosit eldesi ve farklı kalitedeki oositlerin maturasyon oranlarının karşılaştırılması
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Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sunulan çalışmanın amacı; in vitro embriyo üretimi amacıyla mezbahadan temin edilen sığır ovaryumlarından elde edilen oosit sayı ve kalitesinin belirlenmesi ve farklı kalitedeki oositlerin maturasyon oranı arasındaki ilişkinin değerlendirilmesidir. Gereç ve Yöntem: Bu amaçla 370 ovaryum 100.000 IU penicilinstreptomisin içeren %0,9 izotonik sodyum klorür içerisinde 2-4 saatte laboratuvara getirildi. Ovaryumların 40 tanesi kistve adezyon sebebiyle çalışmada kullanılmadı. Geriye kalan 330 ovaryumdan aspirasyon yöntemiyle 1894 adet oosit elde edildi. Elde edilen oositlerin kalitesi sitoplazma yoğunluğu ve oosit çevresindeki kumulus hücrelerinin dağılımına göre A, B, C ve D kalite olarak değerlendirildi. Bunların A ve B kalite olan 1384 adeti ticari bir maturasyon medyumu ile (BO-IVM, Bioscience, UK) 38,8°C’de %5,5 CO2’li ortamda atmosferik O2 ile 24 saat maturasyona bırakıldı. Bulgular: Aspirasyon yöntemiyle 800 adet A, 584 adet B, 450 adet C ve 60 adet D kalite olmak üzere toplam 1894 adet oosit elde edildi. Ovaryum başına ise 2,42 adet A, 1,76 adet B, 1,36 adet C ve 0,18 adet D kalite oosit düştüğü tespit edildi. Maturasyon için sadece A ve B kalitede olan oositler kullanıldı. Bunların maturasyon oranları değerlendirildiğinde A kalite oositlerde %90,3 ve B kalite oositlerde ise %83,3 olarak belirlendi(p?0,01). Çalışmanın toplam maturasyon oranı ise %87,4 olarak tespit edildi. Öneri: Sonuç olarak maturasyon oranlarının oosit kalitesine göre değiştiği ve kalitenin düşmesi ile maturasyon oranlarının azaldığı tespit edilmiştir. Bu nedenle maturasyon öncesi oosit kalitesinin de- ğerlendirilmesinin gerekli olduğu kanısına varılmıştır.
Aim: The aim of the presented study; oocyte number and quality obtained from bovine ovaries procured from abattoir and evaluation of the relationship between different quality oocyte maturation rate for in vitro embryo production. Materials and Methods: For this purpose, 370 ovaries were transported with 0.9% isotonic sodium chloride containing 100,000 IU penicilin-streptomycin and brought to the laboratory within 2-4 hours. 40 of the ovaries were not used in the study due to their cyst and adhesion nature. 1894 oocytes were obtained from the remaining 330 ovaries by aspiration method. The quality of the obtained oocytes was evaluated as A, B, C and D quality according to the cytoplasm density and the distribution of the cumulus cells around the oocyte. 1384 of them, A and B quality, were left to maturation with a commercial maturation medium (BO-IVM,Bioscience, UK)at 38.8 °C with atmospheric O2 at s5.5% CO2 for 24 hours. Results: With the aspiration method, including 800 A, 584 B, 450 C and 60 D quality a total of 1894 oocytes were obtained. In addition, it was determined that the number of 2.42 A, 1.76 B, 1.36 C and 0.18 D per ovary was reduced. Only A and B quality oocytes were used for maturation. The maturation rate was determined as 90.3% in A quality oocytes and 83.3% in B quality oocytes (p?0.01). The total maturation rate of the study was determined as 87.4%. Conclusion: As a result, it was determined that maturation rates change according to oocyte quality and maturation rates reduce with the decrease in quality. Therefore, it was concluded that it is necessary to evaluate the oocyte quality before maturation.
Aim: The aim of the presented study; oocyte number and quality obtained from bovine ovaries procured from abattoir and evaluation of the relationship between different quality oocyte maturation rate for in vitro embryo production. Materials and Methods: For this purpose, 370 ovaries were transported with 0.9% isotonic sodium chloride containing 100,000 IU penicilin-streptomycin and brought to the laboratory within 2-4 hours. 40 of the ovaries were not used in the study due to their cyst and adhesion nature. 1894 oocytes were obtained from the remaining 330 ovaries by aspiration method. The quality of the obtained oocytes was evaluated as A, B, C and D quality according to the cytoplasm density and the distribution of the cumulus cells around the oocyte. 1384 of them, A and B quality, were left to maturation with a commercial maturation medium (BO-IVM,Bioscience, UK)at 38.8 °C with atmospheric O2 at s5.5% CO2 for 24 hours. Results: With the aspiration method, including 800 A, 584 B, 450 C and 60 D quality a total of 1894 oocytes were obtained. In addition, it was determined that the number of 2.42 A, 1.76 B, 1.36 C and 0.18 D per ovary was reduced. Only A and B quality oocytes were used for maturation. The maturation rate was determined as 90.3% in A quality oocytes and 83.3% in B quality oocytes (p?0.01). The total maturation rate of the study was determined as 87.4%. Conclusion: As a result, it was determined that maturation rates change according to oocyte quality and maturation rates reduce with the decrease in quality. Therefore, it was concluded that it is necessary to evaluate the oocyte quality before maturation.
Açıklama
Anahtar Kelimeler
Sığır, oosit kalitesi, maturasyon, Bovine, oocyte quality, maturation
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
37
Sayı
1
Künye
Satılmış, F., Güler, M., (2021). Sığırlarda oosit eldesi ve farklı kalitedeki oositlerin maturasyon oranlarının karşılaştırılması. Eurasian Journal of Veterinary Sciences, 37 (1), 9-15. DOI: 10.15312/EurasianJVetSci.2021.320
