A new method in CNS (Central nervous system) in vitro cultures in the mouse: Study of effectiveness

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dc.contributor.authorErdogan, Ender
dc.contributor.authorOzturk, Gurkan
dc.contributor.authorRagbetli, Murat Cetin
dc.date.accessioned2020-03-26T17:46:44Z
dc.date.available2020-03-26T17:46:44Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractIn this study: to evaluate the effectiveness of collagen coating method, using in the peripheral nervous system cultures. and its involving factors caused from manipulations in central nervous system (CNS) cultures was aimed. Via frontal approach, brains, transected from young Swiss albino mice, were taken into artificial cerebro-spinal fluid immediately and made blocks in agarose gel. With a vibration microtome, 200 pm thickness horizontally live slices were taken in to the dishes filled with culture medium. Tissue sections were analyzed as two groups. In the group 1 (control): fresh slices were evaluated directly. In the group 2: sections were covered with collagen gel (Type I) and left in the incubator (5% CO(2)) for 3 days. These sections were dyed with calcein and propidium iodide for viability and non-viability and then observed with confocal laser scanning microscope. Images were captured digitally and examined. Since negative effects of high melting temperature of standard agar on the livability, using low melting agar to tissue blocking and high frequency - low speed vibrotome setting to cut were more preferably. In the 3 days cultures, viability/nonviability rates were indicated better values. It is concluded that, in the CNS slicing cultures, collagen coating method was an easier, effective, useful and alternative method to present techniques.en_US
dc.identifier.citationErdoğan, E., Öztürk, G., Rağbetli M. Ç. (2010). Fare In Vitro Merkezi Sinir Sistemi Kültürlerinde Yeni Bir Metot: Etkinlik Araştırması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(6), 1037-1043.
dc.identifier.endpage1043en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue6en_US
dc.identifier.startpage1037en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24531
dc.identifier.volume16en_US
dc.identifier.wosWOS:000285216200023en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorErdogan, Ender
dc.language.isotren_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMiceen_US
dc.subjectCentral nervous systemen_US
dc.subjectCultureen_US
dc.subjectSlice sectionen_US
dc.subjectConfocal microscopeen_US
dc.titleA new method in CNS (Central nervous system) in vitro cultures in the mouse: Study of effectivenessen_US
dc.typeArticleen_US

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