A Comparison of Immuncapture Agglutination and ELISA Methods in Sero-logical Diagnosis of Brucellosis

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dc.contributor.authorOzdemir, Mehmet
dc.contributor.authorFeyzioglu, Bahadir
dc.contributor.authorKurtoglu, Muhammed Guzel
dc.contributor.authorDogan, Metin
dc.contributor.authorDagi, Hatice Turk
dc.contributor.authorYuksekkaya, Serife
dc.contributor.authorKesli, Recep
dc.date.accessioned2020-03-26T18:08:08Z
dc.date.available2020-03-26T18:08:08Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and im-muncapture agglutination tests with Coombs anti-brucella test. Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were in-cluded into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Im-muncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated. Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test. Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.en_US
dc.description.sponsorshipSelcuk University School of MedicineSelcuk Universityen_US
dc.description.sponsorshipThis study was supported by a scientific research fund of Selcuk University School of Medicine.en_US
dc.identifier.endpage432en_US
dc.identifier.issn1449-1907en_US
dc.identifier.issue5en_US
dc.identifier.pmid21814476en_US
dc.identifier.startpage428en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26040
dc.identifier.volume8en_US
dc.identifier.wosWOS:000292520000012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIVYSPRING INT PUBLen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF MEDICAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBrucellosisen_US
dc.subjectimmuncaptureen_US
dc.subjectELISAen_US
dc.subjectIgGen_US
dc.subjectIgMen_US
dc.subjectserologic diagnosisen_US
dc.titleA Comparison of Immuncapture Agglutination and ELISA Methods in Sero-logical Diagnosis of Brucellosisen_US
dc.typeArticleen_US

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