Immunohistochemical and immunocytochemical findings associated with Marek's disease virus in naturally infected laying hens

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dc.contributor.authorYavuz, O.
dc.contributor.authorErer, H.
dc.date.accessioned2020-03-26T19:41:51Z
dc.date.available2020-03-26T19:41:51Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractWe compared immunohistochemical (IHC) staining of tissue sections of liver, kidney, spleen, lung, proventriculus, sciatic nerve, bursa of Fabricius, brain, heart, intestine and skin; immunocytochemical (ICC) staining of peripheral blood samples and touch preparations of liver, spleen and kidney of laying hens naturally infected with Marek's disease (MD) virus. We used one hundred and fifty 5-17-week-old commercial hens. IHC and ICC staining were performed using polymer-based techniques. IHC staining exhibited mostly free immunopositive reactions in tumor cells and in the cytoplasm of the parenchymal cells of liver, kidney, spleen and bursa of Fabricius. In the sciatic nerve, severe reactions were observed in the cytoplasm of plasma and MD cells in the lymphoproliferative areas. Pronounced staining was found in the lymphoid cells in the medulla of intrafollicular regions in the bursa of Fabricius. Although immunostaining was observed in the liver and spleen touch preparations, there was no staining in the kidneys and peripheral blood cell samples. The presence of virus in the tissue and peripheral blood samples and in touch preparations was compared immunohistochemically and immunocytochemically. IHC and ICC techniques were helpful for diagnosis of MD. Peripheral blood samples are inappropriate for field conditions and natural infections.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Committee [10102022]en_US
dc.description.sponsorshipThis study funded by Selcuk University Scientific Research Projects Committee, project number 10102022.en_US
dc.identifier.doi10.1080/10520295.2017.1359750en_US
dc.identifier.endpage505en_US
dc.identifier.issn1052-0295en_US
dc.identifier.issn1473-7760en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage498en_US
dc.identifier.urihttps://dx.doi.org/10.1080/10520295.2017.1359750
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35181
dc.identifier.volume92en_US
dc.identifier.wosWOS:000424145900005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofBIOTECHNIC & HISTOCHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjecthensen_US
dc.subjectimmunocytochemistryen_US
dc.subjectimmunohistochemistryen_US
dc.subjectMarek's diseaseen_US
dc.subjecttumoren_US
dc.titleImmunohistochemical and immunocytochemical findings associated with Marek's disease virus in naturally infected laying hensen_US
dc.typeArticleen_US

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