Glucose biosensor based on immobilization of glucose oxidase in electropolymerized poly(o-phenylenediamine) film on platinum nanoparticles-polyvinylferrocenium modified electrode

Basit Öğe Kaydı
dc.contributor.authorTurkmen, Esra
dc.contributor.authorBas, Salih Zeki
dc.contributor.authorGulce, Handan
dc.contributor.authorYildiz, Salih
dc.date.accessioned2020-03-26T18:50:55Z
dc.date.available2020-03-26T18:50:55Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA new biosensor based on immobilization of glucose oxidase (Gox) using enzyme solution containing o-phenylenediamine(oPD) on platinum nanoparticles (PtNPs) electrodeposited polyvinylferrocenium perchlorate matrix (PVF+ClO4-) was fabricated for the bioelectrochemical determination of glucose. Firstly, PVF+ClO4- film was electroprecipitated on Pt electrode by electrooxidation of polyvinylferrocene, and then PtNPs were electrodeposited on PVF+ClO4-/Pt. Finally, Gox was immobilized simultaneously with the electropolymerization of oPD on PtNPs/PVF+ClO4-/Pt. The biosensor showed excellent anti-interference ability to ascorbic acid and uric acid due to the permselectivity of PoPD in the structure of the proposed biosensor. The linear range of Gox-PoPD/PtNPs/PVF+ClO4-/Pt was from 0.06 mM to 9.64 mM with a sensitivity of 17.40 mu A mM(-1) cm(-2) and a detection limit of 0.018 mM. The effects of applied potential, PVF+ClO4- film thickness, oPD concentration, Gox concentration, electropolymerization time and temperature on the response current of the biosensor were investigated in detail. The electrocatalytic effect of PtNPs as well as repeatability, reproducibility and stability of the biosensor were also studied. PtNPs modified PVF+ClO4-/Pt exhibited excellent electrocatalytic activity to the electrooxidation of H2O2. In addition, the biosensor was successfully applied for the determination of glucose concentration in human blood serum samples. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipSelcuk UniversitySelcuk Universityen_US
dc.description.sponsorshipThis work was financially supported by the Scientific Research Projects of Selcuk University.en_US
dc.identifier.doi10.1016/j.electacta.2013.12.189en_US
dc.identifier.endpage102en_US
dc.identifier.issn0013-4686en_US
dc.identifier.issn1873-3859en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage93en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.electacta.2013.12.189
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30893
dc.identifier.volume123en_US
dc.identifier.wosWOS:000334898800012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.relation.ispartofELECTROCHIMICA ACTAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGlucose biosensoren_US
dc.subjectPoly(o-phenylenediamine)en_US
dc.subjectPlatinum nanoparticlesen_US
dc.subjectPolyvinylferroceneen_US
dc.subjectElectrodepositionen_US
dc.titleGlucose biosensor based on immobilization of glucose oxidase in electropolymerized poly(o-phenylenediamine) film on platinum nanoparticles-polyvinylferrocenium modified electrodeen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu