Efficacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in mice

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dc.contributor.authorErganis, Osman
dc.contributor.authorHadimli, Hasan Huseyin
dc.contributor.authorSayin, Zafer
dc.contributor.authorSakmanoglu, Asli
dc.contributor.authorPinarkara, Yasemin
dc.contributor.authorOzdemir, Ozgur
dc.date.accessioned2020-03-26T19:05:54Z
dc.date.available2020-03-26T19:05:54Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to determine the efficacy of inactive Rhodococcus equi vaccine candidates included that bacterin+aluminum hydroxide Al(OH)(3)), bacterin+VapA+(Al(OH)(3)), bacterin+Montanide IMS 3012 (IMS), bacterin+ VapA+IMS, and live vaccine using mice as a model. The efficacy of vaccine was evaluated according to clinical findings, humoral and cellular immunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrificed mice. Inactive R. equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injected once. Fifteen days after the last vaccination, aerosol challenges were carried out with the pathogenic R. equi VapA(+)K2002 strain in all groups. Two mice were sacrificed from each challenge groups on days 1, 3, 5, and 7. The antibody titers of vaccinated mice were found to be significantly higher than those of the controls. The largest number of INF-g positive samples were detected in the bacterin+ VapA+IMS and bacterin+ IMS groups. IL-4 positivity was determined only in live vaccine groups. The lowest reisolation rate of R. equi from internal organs was observed in the bacterin+VapA+IMS group. It was concluded that R. equi vaccines, and especially bacterin+VapA+IMS, are useful to protect mice against R. equi infection.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK Project)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108G030]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK Project No. 108G030).en_US
dc.identifier.doi10.3906/vet-1410-44en_US
dc.identifier.endpage301en_US
dc.identifier.issn1300-0128en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage295en_US
dc.identifier.urihttps://dx.doi.org/10.3906/vet-1410-44
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32091
dc.identifier.volume39en_US
dc.identifier.wosWOS:000356079900007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAKen_US
dc.relation.ispartofTURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRhodococcus equien_US
dc.subjectvaccineen_US
dc.subjectmice modelen_US
dc.subjectVapAen_US
dc.subjectMontanide IMS 3012en_US
dc.subjectAl(OH)(3)en_US
dc.titleEfficacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in miceen_US
dc.typeArticleen_US

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