Diagnosis of Bovine Tuberculosis by PPD-ELISA and Sonication-ELISA

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dc.contributor.authorSayin, Z.
dc.contributor.authorErganis, O.
dc.date.accessioned2020-03-26T18:41:30Z
dc.date.available2020-03-26T18:41:30Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractMycobacterium bovis (M. bovis) infection in cattle remains a major zoonotic and economic problem in many countries. In addition, M. bovis is an important pathogen of both man and animals. The standard diagnostic test for this disease is the intradermal tuberculin test, one of the oldest immunological tests still in widespread use. However, this test can lack both sensitivity and specificity. Serological tests such as ELISA (Enzyme Linked Immunosorbent Assay) have been suggested as an alternative method for the diagnosis of tuberculosis. In the present study, two serum ELISA's, performed for the detection of M. bovis-specific antibodies, were evaluated against the culture of tissue samples from 135 slaughtered dairy cows, which were intradermal tuberculin-test-positive. ELISA plates were coated with Bovine Purified Protein Derivative (PPD B) and M. bovis-sonication antigen. Samples were taken from the bronchial (BLN), mesenteric (MLN) and prescapular (PLN) lymph nodes and tubercules (TB) of slaughtered cattle. Tissue samples were cultured in Lowenstein-Jensen medium. Blood samples, which were collected from the tuberculin-test-positive animals before slaughter, were tested by PPD B-ELISA (PPD-ELISA) and M. bovis sonication-ELISA (sonication-ELISA). Mycobacterium spp. was isolated from 77 out of 135 cattle. Out of 135 cattle, 62 (45.9%) and 43 (31.8%) were determined to be positive for PPD-ELISA and sonication-ELISA, respectively. Using culture-positive animals as a gold standard, the sensitivity and specificity of these two methods were detected as 48.2%-55.2% and 31.2%-44.5%, respectively.en_US
dc.description.sponsorshipSelcuk University [SUBAPK Konya, Turkey]Selcuk University [07102007]en_US
dc.description.sponsorshipThis work was supported by the Coordinatorship for Scientific Research Projects of Selcuk University [SUBAPK (No: 07102007) Konya, Turkey]. This article forms part of a PhD thesis. This research has been approved by Ethical Committee of Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey (Ethical Committee No: 2006-078). The abstract was presented to XXVII World Buiatrics Congress 2012, Lisbon, Portugal. The abstract was published in the proceeding book.en_US
dc.identifier.endpage184en_US
dc.identifier.issn0334-9152en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage180en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29378
dc.identifier.volume68en_US
dc.identifier.wosWOS:000324439400008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherISRAEL VETERINARY MEDICAL ASSOCen_US
dc.relation.ispartofISRAEL JOURNAL OF VETERINARY MEDICINEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMycobacterium bovisen_US
dc.subjectPPD-ELISAen_US
dc.subjectsonication-ELISAen_US
dc.subjectbovine tuberculosisen_US
dc.titleDiagnosis of Bovine Tuberculosis by PPD-ELISA and Sonication-ELISAen_US
dc.typeArticleen_US

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