Central composite design and response surface methodology for the optimization of Ag+-HPLC/ELSD method for triglyceride profiling

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dc.contributor.authorArslan, Fatma Nur
dc.contributor.authorKara, Huseyin
dc.date.accessioned2020-03-26T19:34:14Z
dc.date.available2020-03-26T19:34:14Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe study presents the application of central composite design (CCD) and response surface methodology (RSM) for the optimization of silver-ion normal phase HPLC/ELSD (Ag+-HPLC/ELSD) method parameters to profile the isomers of triglycerides in vegetable oils. The significance of a second-order polynomial model for predicting the optimal values of Ag+-HPLC/ELSD method parameters was evaluated by the analysis of variance, ANOVA, and 3D response surface plots for the interactions between three variables were constructed. Three experimental parameters were chosen as independent variables which are the flow rate of mobile phase, temperature of column compartment and concentration of sample. A multivariate five-level CCD and RSM were used to confirm a quadratic model as a functional relationship between the response values (Rs, N, alpha and k') and variables. The optimum values of parameters were found to be a flow rate of 1.25 mL min(-1), temperature of column compartment of 20 A degrees C, and sample concentration of 5 x 10(-2) mg A mu L-1. Regression analysis with an R-2 values indicated as an adequate correlation between the experimental and predicted response values. ANOVA test results were also confirm that the models can be successfully used to predict the optimum parameters of Ag+-HPLC/ELSD method. Therefore, the proposed model provides an efficient, automated, and robust Ag+-HPLC/ELSD method for triglyceride profiling and is also suitable for a number of applications and analytical method developments for vegetable oils.en_US
dc.description.sponsorshipTUBITAK projectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112T373]; Selcuk University Coordinators of Scientific ResearchSelcuk University [12401045, 11201063]; Karamanoglu Mehmetbey University Scientific Research Project Centre [14-M-16]en_US
dc.description.sponsorshipThe present study is a part of Ph.D thesis entitled "Integration of retarded and pre-concentrated SPE systems to FIA-HPLC systems for vegetable oil analysis and improvement of its applications". The research was supported by TUBITAK project with 112T373 project number and Selcuk University Coordinators of Scientific Research with 12401045 and 11201063 project numbers, and Karamanoglu Mehmetbey University Scientific Research Project Centre with 14-M-16 project number.en_US
dc.identifier.doi10.1007/s11694-016-9462-5en_US
dc.identifier.endpage912en_US
dc.identifier.issn2193-4126en_US
dc.identifier.issn2193-4134en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage902en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11694-016-9462-5
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34864
dc.identifier.volume11en_US
dc.identifier.wosWOS:000400385000057en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofJOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATIONen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSilver ion high performance liquid chromatographyen_US
dc.subjectVegetable oilen_US
dc.subjectResponse surface methodologyen_US
dc.subjectEvaporative light scattering detectoren_US
dc.subjectAnalysis of varianceen_US
dc.titleCentral composite design and response surface methodology for the optimization of Ag+-HPLC/ELSD method for triglyceride profilingen_US
dc.typeArticleen_US

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