Isolation of Ornithobacterium Rhinotracheale From Chickens and Turkeys

A total of 251 tracheal swabs and a total of 102 tracheae, lungs, airsacs, livers and spleens were collected from commercial layer, broiler and broiler parent stock flocks with respiratory infection. From turkey flocks, 96 tracheal swabs and 5 tissue samples from the trachea, lung, airsac, liver and spleen were also taken. Cultivations on Blood Agar supplemented with 51 10%sheep blood, on Chocolate Agar and on MacConkey Agar were incubated at 37degreesC for 24-72 hours in the presence of 10%CO(2)Gram staining was carried out for the smooth and non-hemolytic colonies 1-3 mm in diameter and for those showing grayish pigmentation after approximately 48 hours of culture. Cram negative pleomorphic rods were considered to be Ornithobacterium rhinotracheale (ORT) and other conventional tests were made as follows: hemolysis, motility, spore formation, growth on MacConkey and TSI agars, catalase, indol, sorbitol, inositol, rafinose, trehalose, xylose, dulcitol, nitrate, methyl red, oxidase, beta-D galactosidase, urease, glucose, galactose, lactose, maltose, fructose and Voges-Proskauer. ORT was isolated from none of the samples taken from turkeys, broiler or broiler parent stock flocks. However, two ORT isolations were achieved from two commercial pullets aged 12 and 15 weeks. One of these isolates was serotyped as "B" by the ACP test while the other was not found to belong to any of the serogroups. Both of the isolates were found to be susceptible to oflaxacin, erythromicin, lincomycin, amoxicillin, and amoxicillin+clavulonic acid by antibiotic suspectibility tests.

Solunum sistemi enfeksiyonu bulunan yumurtac› piliç, broyler ve broyler dam›zl›k kümeslerinden 251 adet trakeal s›vap ile 102 adet trakea, akci¤er, hava kesesi, karaci¤er ve dalak, hindi kümeslerinden ise 96 adet trakeal s›vap ile 5 adet trakea, akci¤er, hava kesesi, karaci¤er ve dalak örne¤i ile çal›fl›ld›. Örnekler %5-10 koyun kanl› agar, çukulata agar ve MacConkey agara ekilerek 37ºC’de 24-72 saat %10 CO2’li ortamda inkübe edildi. Yaklafl›k 48 saat sonra, 1-3 mm çap›nda, düzgün kenarl›, opak-gri pigmentli, hemoliz yapmayan kolonilere Gram boyama yap›ld›. Gram negatif pleomorfik basillerin kültürel ve biyokimyasal özellikleri incelendi. Hemoliz, hareket, spor oluflumu, MacConkey ve TSI agarda üreme, katalaz, jelatinaz, indol, sorbitol, inositol, rafinoz, trehaloz, ksiloz, dulsitol, nitrat, metil red, oksidaz, β-D galaktosidaz, üreaz, glikoz, galaktoz, laktoz, maltoz, fruktoz ve Voges-Proskauer özelliklerine göre Ornithobacterium rhinotracheale (ORT) olarak identifiye edildi. Hindi, broyler ve broyler dam›zl›klardan al›nan örneklerden ORT izolasyonu yap›lamazken, 12 ve 15 haftal›k yumurtac› piliç kümeslerinden al›nan 2 trakeal s›vap örne¤inden ORT izole edildi. AGP ile biri serotip B olarak serotiplendirilirken, di¤erinin serotipi belirlenemedi. ORT izolatlar› antibiyotik duyarl›l›k testlerinde; oflaksasin, eritromisin, linkomisin, amoksisilin ve amoksisilin+klavulonik asit’e duyarl› bulundu.