Sperm superoxide dismutase is associated with bull fertility

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dc.contributor.authorGrant, Kamilah E.
dc.contributor.authorde Oliveira, Rodrigo V.
dc.contributor.authorHennington, Bettye Sue
dc.contributor.authorGovindaraju, Aruna
dc.contributor.authorPerkins, Andy
dc.contributor.authorStokes, John
dc.contributor.authorRowe, Dennis
dc.date.accessioned2020-03-26T19:26:33Z
dc.date.available2020-03-26T19:26:33Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractDecreasing mammalian fertility and sperm quality have created an urgent need to find effective methods to distinguish non-viable from viable fertilising spermatozoa. The aims of the present study were to evaluate expression levels of beta-tubulin 2C (TUBB2C), heat shock protein 10 (HSP10), hexokinase 1 (HXK1) and superoxide dismutase 1 (SOD1) in spermatozoa from Holstein bulls with varying fertility using western blotting and to analyse the biological networks of these key sperm proteins using a bioinformatics software (Metacore; Thomson-Reuters, Philadelphia, PA, USA). The rationales behind this study were that the sperm proteins play crucial roles in fertilisation and early embryonic development in mammals and ascertaining the biological networks of the proteins helps us better understand sperm physiology and early mammalian development. The results showed that expression of SOD1 was higher in spermatozoa from high fertility bulls (P< 0.05) and that SOD1 is the best protein to diagnose bulls based on the fertility index (P< 0.05). Using Metacore analysis, we identified an SOD1 network with pathways and linkages with other relevant molecules. We concluded that SOD1 sperm expression is associated with in vivo bull fertility. The findings are important because they illuminate molecular and cellular determinants of sperm viability and the identified protein markers can be used to determine bull fertility.en_US
dc.description.sponsorshipMississippi Agricultural and Forestry Experiment station (USA); National Council of Technological and Scientific Development (CNPq)National Council for Scientific and Technological Development (CNPq); Coordination for the Improvement of Higher Education Personnel (Capes-Brazil)CAPES; Fulbright (USA); Alta Genetics Inc. (USA)en_US
dc.description.sponsorshipThis study was funded by the Mississippi Agricultural and Forestry Experiment station (USA), National Council of Technological and Scientific Development (CNPq), Coordination for the Improvement of Higher Education Personnel (Capes-Brazil), Fulbright (USA) and Alta Genetics Inc. (USA).en_US
dc.identifier.doi10.1071/RD14399en_US
dc.identifier.endpage1413en_US
dc.identifier.issn1031-3613en_US
dc.identifier.issn1448-5990en_US
dc.identifier.issue9en_US
dc.identifier.pmid25896743en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1405en_US
dc.identifier.urihttps://dx.doi.org/10.1071/RD14399
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34022
dc.identifier.volume28en_US
dc.identifier.wosWOS:000381302100017en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCSIRO PUBLISHINGen_US
dc.relation.ispartofREPRODUCTION FERTILITY AND DEVELOPMENTen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectproteinen_US
dc.subjectreproductionen_US
dc.subjectsemenen_US
dc.titleSperm superoxide dismutase is associated with bull fertilityen_US
dc.typeArticleen_US

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