Heterologous expression of glutamyl-tRNA reductase gene in Rhodobacter sphaeroides O.U.001 to enhance 5-aminolevulinic acid production

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dc.contributor.authorKars, Gökhan
dc.contributor.authorAlparslan, Ümmühan
dc.date.accessioned2020-03-26T18:50:57Z
dc.date.available2020-03-26T18:50:57Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe pathways for synthesis of 5-aminolevulinic acid (5-ALA) use either succinyl-CoA and glycine (C-4 pathway), or glutamate (C-5 pathway). Although Rhodobacter sphaeroides synthesizes 5-ALA through the C-4 pathway, it also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. The glutamyl-tRNA reductase gene was cloned from Rhodospirillum rubrum and expressed in R. sphaeroides; thus, the C-5 pathway was enabled to function upon assembling all the required genes. Consequently, a new and unique bacterial strain producing more 5-ALA was developed. Biohydrogen was also produced in the same bioprocess within a biorefinery approach using sugar beet molasses as substrate. The amount of 5-ALA produced by the modified strain was 25.9mg/g dry cell weight (DCW), whereas the wild-type strain produced 12.4mg/g DCW. In addition, the amount of H-2 generated by the modified and wild-type cells, respectively, was 0.92 L/L culture and 1.05 L/L culture.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T523]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey [grant number 111T523].en_US
dc.identifier.doi10.1080/13102818.2014.978170en_US
dc.identifier.endpage1041en_US
dc.identifier.issn1310-2818en_US
dc.identifier.issn1314-3530en_US
dc.identifier.issue6en_US
dc.identifier.pmid26740781en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1034en_US
dc.identifier.urihttps://dx.doi.org/10.1080/13102818.2014.978170
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30901
dc.identifier.volume28en_US
dc.identifier.wosWOS:000348931600007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofBIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENTen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectheterologous expressionen_US
dc.subjectglutamyl-tRNA reductaseen_US
dc.subjectaminolevulinic aciden_US
dc.subjectbiohydrogenen_US
dc.subjectRhodobacter sphaeroides O.U.001en_US
dc.titleHeterologous expression of glutamyl-tRNA reductase gene in Rhodobacter sphaeroides O.U.001 to enhance 5-aminolevulinic acid productionen_US
dc.typeArticleen_US

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