Investigation of autophagic effects of melatonin on breast cancer stem cells

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dc.contributor.authorDonmez, Huseyin
dc.contributor.authorKocak, Nadir
dc.contributor.authorYildirim, Ibrahim Halil
dc.date.accessioned2020-03-26T19:41:56Z
dc.date.available2020-03-26T19:41:56Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAutophagy plays important roles in physiologic cellular events and also in cancers. It has been reported that cells escaped from death via autophagy and if autophagy inhibited, cells conducted to apoptosis. In some studies, protective effects of the melatonin on induced autophagy in cancer cells reported. In this study, we aimed to evaluate the autophagic effect of melatonin in cancer stem cells. For this purpose, CD44+/CD24-phenotype cells sorted from melatonin-treated and untreated MCF-7 and HEK293 cells. Effect of melatonin on autophagy was analysed by immunofluorescence and western blot analysis. Results showed that melatonin-induced LC3 (Microtubule-associated protein 1A/1B-light chain 3) aggregation and formation of autophagic vacuoles in MCF-7 derived cancer stem cells and also induced LC3-I to LC3-II conversion in these stem cells when compared to untreated control group. In conclusion, melatonin showed a pro-autophagic effect in CD44+/CD24-stem cells derived from MCF-7 cells and anti-autophagic effect in CD44+/CD24-stem cells obtained from HEK293 cells.en_US
dc.identifier.endpage5053en_US
dc.identifier.issn0970-938Xen_US
dc.identifier.issn0976-1683en_US
dc.identifier.issue11en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage5048en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35221
dc.identifier.volume28en_US
dc.identifier.wosWOS:000407324200060en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherALLIED ACADen_US
dc.relation.ispartofBIOMEDICAL RESEARCH-INDIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAutophagyen_US
dc.subjectMCF-7en_US
dc.subjectHEK293en_US
dc.subjectMelatoninen_US
dc.subjectCancer stem cellsen_US
dc.titleInvestigation of autophagic effects of melatonin on breast cancer stem cellsen_US
dc.typeArticleen_US

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