Screening of PTEN gene mutations in gastric cancer
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: It is not exactly defined how many known gastric cancer causative factors cause cancer in stomach lining cells. PTEN gene is an important tumor suppressor gene that is frequently activated in many cancer cells. In this study, we aimed to investigate the molecular changes of PTEN gene in patients with gastric adenocarcinoma. Material and Methods: Forty seven paraffin-embedded tumor tissues from patients diagnosed with gastric adenocarcinoma and adjacent normal tissues were screened for frequency of PTEN mutations using polymerase chain reaction-single strand conformation polymorphism and high resolution melting curve analyses, and DNA sequencing. Results: There were no mutations in exons 3,4,5,6 in the PTEN gene. However, in 8 patients, a single-nucleotide polymorphism (rs1903858) was revealed by sequencing in the junction of intron 1-exon 2, and its clinical significance is not known. Conclusion: There are no data about the function and frequency of this single-nucleotide polymorphism in gastric cancer patients. Therefore, single-nucleotide polymorphism seen in gastric cancer should be evaluated in a larger population of patients to determine its frequency and function in this group. © 2014 by Türkiye Klinikleri.
Açıklama
Anahtar Kelimeler
DNA, Polymorphism, PTEN phosphohydrolase, Sequence analysis, Single nucleotide, Stomach neoplasms
Kaynak
Turkiye Klinikleri Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
34
Sayı
1
