Detection of argyrophil nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) in epithelial skin tumours from domestic animals

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dc.contributor.authorHatipoglu, F.
dc.contributor.authorOzdemir, O.
dc.contributor.authorKiran, M. M.
dc.date.accessioned2020-03-26T17:38:10Z
dc.date.available2020-03-26T17:38:10Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe objective of this study was to compare the chemical characterization of the AgNOR bodies and the immunohistochemical detection of the PCNA for differentiating benign and malignant epithelial tumours (n = 21) front various animal Species (mainly dogs and cattle). Fibropapillomas / papillomas (n = 3) and fibropapillomatosis / papillomatosis (n = 3) in cattle. and perianal gland adenomas (n = 3), keracanthomas (n = 2) and epithelioma (n = 1) in dogs constituted the group of benign tumours whereas the identified malignant tumours were squamous cell carcinomas (n = 7) in cattle and in one sheep and carcinomas in dogs (n = 2). A complete AgNOR characterization with determination of the NAI (nuclear area index), the AgNAI (AgNOR area index). the AgNCI (AgNOR count index). the AgNAI/NAI (relative AgNOR area), the AgNAI/AgNCI (mean AgNOR area) and the AgNCI/NAI (A,,NOR density) was performed and PCNA expression was, explored in parallel by immunohistochemistry. Significant increases of AgNAI, AgNCI and AgNAI/NAI were evidenced in malignant tumours compared to benign ones whereas no significant difference of the PCNAI (numbers of PCNA positive cells) was found. Significant and positive correlations found between NAI and AgNAI or mean AgNOR area and between AgNAI and the mean AgNOR area or the relative AgNOR area in both malignant and benign tumours suggest that the AgNOR size is an important parameter for cell proliferation. By contrast, the lack of correlation between AgNCI and the relative AgNOR area and between the AgNOR density and NAI or AgNCI or AgNAI/AgNCI in malignant tumours contrary to benign tumours would be Clue to the under-estimation of the enumeration of the AgNOR bodies in malignant tumours mainly regrouped as AgNOR clusters.en_US
dc.description.sponsorshipCoordinatorship of Selcuk University Scientific ResearchSelcuk University [05401029]; Agriculture, Forestry & Veterinary Research Grant Committee [105 0 693]; Scientific & Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipWe gratefully respect and acknowledge Prof. Dr. Metin M. Kiran died of cancer, for the planning and designing of this study when he was among us. This study was supported by Coordinatorship of Selcuk University Scientific Research Project (Project No: 05401029), the Short-Term R&D Funding Program of Agriculture, Forestry & Veterinary Research Grant Committee (TOVAG -Project No: 105 0 693), and the Scientific & Technological Research Council of Turkey (TUBITAK).en_US
dc.identifier.endpage483en_US
dc.identifier.issn0035-1555en_US
dc.identifier.issn2258-0646en_US
dc.identifier.issue10en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage477en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23396
dc.identifier.volume160en_US
dc.identifier.wosWOS:000272099400007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherECOLE NATIONALE VETERINAIRE TOULOUSEen_US
dc.relation.ispartofREVUE DE MEDECINE VETERINAIREen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSkinen_US
dc.subjectepithelial tumouren_US
dc.subjectmalignancyen_US
dc.subjectAgNORen_US
dc.subjectPCNAen_US
dc.subjectdomestic animalen_US
dc.titleDetection of argyrophil nucleolar organizer regions (AgNORs) and proliferating cell nuclear antigen (PCNA) in epithelial skin tumours from domestic animalsen_US
dc.typeArticleen_US

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