Cell death induced by eluates from hypoallergenic denture base acrylic resins in NIH-3T3 fibroblast cells

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dc.contributor.authorKansu, Gulay
dc.contributor.authorKalyoncuoglu, Tugba
dc.contributor.authorUyar, Pembegul
dc.contributor.authorUzun, Esra
dc.date.accessioned2020-03-26T18:49:40Z
dc.date.available2020-03-26T18:49:40Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground/purpose: The use of a hypoallergenic denture base material for dental patients is susceptible to trigger allergic reactions. The aim of this study was to compare the cytotoxicity at the cellular level of hypoallergenic denture base materials and polymethyl methacrylate denture base materials polymerized in different ways. Materials and methods: Comparisons were done with the MTT (3-4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide) results of eluates, which were derived from all test materials by immersing for 24 and 48 hours in various ratios of the test materials' weights to the volume of Dulbecco's modified Eagle's medium without supplements (0.2, 0.4, and 0.8 g/mL) and incubating NIH-3T3 cells for 24 and 48 hours (N = 9). Then the apoptotic effects of the materials were detected, using the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay. Results: The 24-hour eluates of all materials exhibited strong cytotoxicity, and cytotoxicity increased with time but the difference was not statistically significant. In terms of cell death rates, the materials were ranked as follows: QC 20, Puran HC, Acron MC, Promysan Star. As a result of the apoptotic index of the materials, the ratio of necrotic cells was found from highest to lowest: QC 20, Puran HC, Acron MC, and Promysan Star. Conclusion: It was determined that the test materials have cytotoxic effects on fibroblasts, and polymethyl methacrylate-based test materials have a more necrotic effect on cells; however, other test materials induced apoptosis at a higher range. Copyright (C) 2013, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved.en_US
dc.description.sponsorshipFaculty of Dentistry fund, Ankara University, Ankara, TurkeyAnkara University [2006-6]en_US
dc.description.sponsorshipThe authors thank Professor Dr Mesude Iscan (METU, Biological Science, Ankara, Turkey) for providing excellent working conditions at her laboratory and great attributions. The authors also thank Mr Antonio Pedrazzini for valuable support in the preparation of test samples from Promysan. This work was supported by the Faculty of Dentistry fund (2006-6), Ankara University, Ankara, Turkey.en_US
dc.identifier.doi10.1016/j.jds.2013.01.008en_US
dc.identifier.endpage387en_US
dc.identifier.issn1991-7902en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage381en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.jds.2013.01.008
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30662
dc.identifier.volume9en_US
dc.identifier.wosWOS:000347131400011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER TAIWANen_US
dc.relation.ispartofJOURNAL OF DENTAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectacrylic denture base materialsen_US
dc.subjectapoptosisen_US
dc.subjectcytotoxicityen_US
dc.subjectMTT testen_US
dc.subjectnecrosisen_US
dc.titleCell death induced by eluates from hypoallergenic denture base acrylic resins in NIH-3T3 fibroblast cellsen_US
dc.typeArticleen_US

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