Interaction of donepezil with human serum albumin on amine-modified magnetic nanoparticles

dc.contributor.authorBuzoglu, Leman
dc.contributor.authorMaltas, Esra
dc.contributor.authorOzmen, Mustafa
dc.contributor.authorYildiz, Salih
dc.date.accessioned2020-03-26T18:51:11Z
dc.date.available2020-03-26T18:51:11Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe interaction between the drug donepezil and human serum albumin (HSA) was examined on the surface of amine modified superparamagnetic iron oxide nanoparticles (SPIONs), which were synthesized by the coprecipitation of ferrous and ferric salts with NH4OH and then modified with [3-Aminopropyl] triethoxysilane (APTES) to obtain functional amine groups on the nanoparticles' surface. Albumin's binding capacity to APTES-modified SPIONs was estimated by fluorescence spectroscopy. After HSA was bound to the APTES modified SPIONs, donepezil was interacted with the HSA-SPIONs. The binding capacity of the drug was determined using a calibration curve equation that was drawn using fluorescence spectroscopy at 325 and 387 nm, the excitation and emission wavelengths. Thermodynamic parameters were estimated for the interaction of HSA and donepezil on amine-modified SPIONs. Binding was carried out spontaneously via electrostatic interaction. Binding was also examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE), scanning electron microscopy (SEM) and zeta-potential measurements. (C) 2013 Elsevier B.V. All rights reserved,en_US
dc.description.sponsorshipResearch Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipWe would like to thank The Research Foundation of Selcuk University (BAP) for financial support of this work.en_US
dc.identifier.doi10.1016/j.colsurfa.2013.03.009en_US
dc.identifier.endpage145en_US
dc.identifier.issn0927-7757en_US
dc.identifier.issn1873-4359en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage139en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.colsurfa.2013.03.009
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30937
dc.identifier.volume442en_US
dc.identifier.wosWOS:000331687000019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectDonepezilen_US
dc.subjectHuman serum albuminen_US
dc.subjectFluorescence spectroscopyen_US
dc.titleInteraction of donepezil with human serum albumin on amine-modified magnetic nanoparticlesen_US
dc.typeArticleen_US

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