Kloramfenikol' ün sığır polimorfnükleer lökosit (PMNL) fonksiyonları üzerindeki etkilerinin in vitro ve in vivo araştırılması
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Date
2003
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Abstract
Bu çalışmada kloramfenikolün polimorfnükleer lökosit (PMNL)'lerin fagositoz ve hücre içi öldürme aktiviteleri üzerindeki etkisi araştırıldı. Çalışma, in vitro ve in vivo koşullarda gerçekleştirildi. İn vivo aşamada, 08-13 aylık 9 adet Holstein-Fresian sığır kullanıldı. İn vitro bölümde ise kloramfenikol uygulanmamış hayvanlardan alınan kan örneklerinden izole edilen nötrofiller'in 10, 1-000 ve 4000 mug/ml kloramfenikol ihtiva eden inkübasyon ortamlarında fagositoz ve hücre içi öldürme aktiviteleri belirlendi. Buna göre; 10 ve 1000 mug/ml'de PMNL fonksiyonlarında bir değişiklik görülmezken, 1000 ug/ml'de nötrofil başına düşen fagositik canlı C. albicans sayısında artma, 4000 mug/ml'de ise fonksiyonlarda önemli derecede azalma saptandı. İn vivo çalışmada, 10 mg/kg 12 saat ara ile günde iki kez, beş gün süre ile kloramfenikol uygulandı. Kan örnekleri, sabah uygulamasından 2.5 saat sonra alınarak, plazma kloramfenikol ölçümü ve PMNL izolasyonu yapıldı. PMNL fonksiyonlarında, ilk günden itibaren önemli derecede azalma kaydedildi.
In this study, effects of chloramphenicol on phagocytosis and intracellular killing activity of polymorphonuclear leucocytes (PMNLs) were investigated. This study was performed in vitro and in vivo conditions. Nine Holstein-Fresians cows (8-13 months' ages) were used. Chloramphenicol were tested at 4000, 1000 and 10 mug/ml of incubation mixtures. Chloramphenicol had no effect on PMNL functions at 1000 and 10 mug/ml concentrations. However, chloramphenicol caused significant increase in intracellular number of live C. albicans on per PMNL at the intermediate dose. The activities of PMNLs were also depressed by chloramphenicol at the highest dose. In vivo studies, at 12 hours intervals, each cow were treated by intramuscularly administration with chloramphenicol at recommended dose (10mg/kg bw) for 5 days. Blood samples were collected at 2.5hr after first injection. A significant reduction in the percentage of phagocytosis and intracellular killing activity was observed beginning of the first day.
In this study, effects of chloramphenicol on phagocytosis and intracellular killing activity of polymorphonuclear leucocytes (PMNLs) were investigated. This study was performed in vitro and in vivo conditions. Nine Holstein-Fresians cows (8-13 months' ages) were used. Chloramphenicol were tested at 4000, 1000 and 10 mug/ml of incubation mixtures. Chloramphenicol had no effect on PMNL functions at 1000 and 10 mug/ml concentrations. However, chloramphenicol caused significant increase in intracellular number of live C. albicans on per PMNL at the intermediate dose. The activities of PMNLs were also depressed by chloramphenicol at the highest dose. In vivo studies, at 12 hours intervals, each cow were treated by intramuscularly administration with chloramphenicol at recommended dose (10mg/kg bw) for 5 days. Blood samples were collected at 2.5hr after first injection. A significant reduction in the percentage of phagocytosis and intracellular killing activity was observed beginning of the first day.
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Veterinerlik
Journal or Series
Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences
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Volume
19
Issue
01.Feb
Citation
Baş, A. L., Demet, Ö. (2003). Kloramfenikol' ün Sığır Polimorfnükleer Lökosit (PMNL) Fonksiyonları Üzerindeki Etkilerinin İn Vitro ve İn Vivo Araştırılması. Veteriner Bilimleri Dergisi. Eurasian Journal of Veterinary Sciences, 19(1-2), 73-80.
