Multidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSn profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic properties

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dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorLlorent-Martinez, Eulogio J.
dc.contributor.authorAumeeruddy, Muhammad Zakariyyah
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.contributor.authorAltunoğlu, Yasemin Çelik
dc.contributor.authorUstaoğlu, Buket
dc.contributor.authorÖçal, Mustafa
dc.contributor.authorGürel, Songül
dc.contributor.authorBene, Kouadio
dc.contributor.authorSinan, Kouadio Ibrahime
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2020-03-26T20:15:19Z
dc.date.available2020-03-26T20:15:19Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractCluysophyllum perpulchrum Mildbr. ex Hutch. & Dalziel has been reported to possess several therapeutic properties in African traditional medicine. However, its pharmacological properties have not been fully studied. Herein, we focused on the evaluation of the antioxidant, enzyme inhibitory, antimicrobial and cytotoxic effects of three solvent extracts (ethyl acetate, methanol, and water) of the stem bark and leaves. Polyphenolic components of the extracts were also identified by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). The methanolic stem bark extract possess the highest DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging, ferric and molybdenum (VI) reducing, and the highest inhibitory effect against acetylcholinesterase and butyrylcholinesterase. The aqueous stem bark extract displayed the highest ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) scavenging and cupric reducing power. In contrast, the leaf ethyl acetate extract was the most effective metal chelator and a-amylase inhibitor while the leaf methanolic extract exhibited the highest tyrosinase and alpha-glucosidase inhibition. The highest total phenolic content (131.58 mg GAE/g extract) was recorded from the methanolic stem bark extract while the leaf methanolic extract was the richest in total flavonoid content (30.43 mg RE/g extract). Flavan-3-ols were main group in the methanol extracts. The minimum inhibitiory concentration values for the methanolic stem bark extract against Gram positive and negative bacteria strains ranged between 62.5-125 mu g/ml. Bark extracts of C. petpulchrtun were very effective against Salmonella kentucky, Proteus vulgaris and Staphylococcus aureus at 62.5 mu g/ml. In addition, cytotoxic activity of stem bark samples was evident at lower concentration than those of leaf extracts. Stem bark extracts displayed the highest prevention against to HeLa cell line (IC50 = 264 mu g/mL after 48 h). In light of the above, C. perpulcluum might provide health benefits against oxidative stress and the associated chronic diseases. Nonetheless, the detailed mechanism of action is yet to be further investigated.en_US
dc.description.sponsorshipKastamonu University (Kastamonu University Scientific Projects Research Office) [KUBAP-01/2016-45]en_US
dc.description.sponsorshipThe authors gratefully acknowledge the generous technical and human support of Universidad de Jaen (ICT of Universidad de Jaen (UJA, MINECO, Junta de Andalucia, FEDER) as well as financial support of Kastamonu University (Kastamonu University Scientific Projects Research Office (Project Number KUBAP-01/2016-45).en_US
dc.identifier.citationBaloğlu, M. C., Llorent-Martínez, E. J., Aumeeruddy, M. Z., Mahomoodally, M. F., Altunoğlu, Y. C., Ustaoğlu, B., Öçal, M., Gürel, S., Bene, K., Sinan, K. I., Zengin, G. (2019). Multidirectional Insights on Chrysophyllum perpulchrum Leaves and Stem Bark Extracts: HPLC-ESI-Msn Profiles, Antioxidant, Enzyme Inhibitory, Antimicrobial and Cytotoxic Properties. Industrial Crops and Products, 134, 33-42.
dc.identifier.doi10.1016/j.indcrop.2019.03.066en_US
dc.identifier.endpage42en_US
dc.identifier.issn0926-6690en_US
dc.identifier.issn1872-633Xen_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage33en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.indcrop.2019.03.066
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38016
dc.identifier.volume134en_US
dc.identifier.wosWOS:000471361200005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorSinan, Kouadio Ibrahime
dc.institutionauthorZengin, Gökhan
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofINDUSTRIAL CROPS AND PRODUCTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAfricaen_US
dc.subjectAntioxidanten_US
dc.subjectEnzyme inhibitionen_US
dc.subjectPolyphenolicen_US
dc.subjectAntimicrobialen_US
dc.subjectAnticanceren_US
dc.titleMultidirectional insights on Chrysophyllum perpulchrum leaves and stem bark extracts: HPLC-ESI-MSn profiles, antioxidant, enzyme inhibitory, antimicrobial and cytotoxic propertiesen_US
dc.typeArticleen_US

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