Agrobacterium-Mediated Transformation of Lupinus Mutabilis L. Using Shoot Apical Explants
Yükleniyor...
Dosyalar
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Polish Academy of Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A procedure for regenerating plants of Lupinus mutabilis from shoot apices, from which the leaf primordia and initial cell layer(s) of the apical meristem were removed, has been used to generate transgenic plants following Agrobacterium tumefaciens-mediated gene delivery. Transformation competent cells, from which buds developed, were located at the periphery of the apical meristem. Kanamycin resistant plants were obtained which expressed ?-glucuronidase activity. Integration of the neomycin phosphotransferase II and ?-glucuronidase genes into the genomes of transgenic plants was confirmed by nonradioactive DNA-DNA hybridisation. This is the first report of the generation of transgenic plants in L. mutabilis.
Açıklama
Anahtar Kelimeler
Agrobacterium tumefaciens, Lupinus mutabilis L. (Lupin), Shoot apices, Transformation, Transgenic plants
Kaynak
Acta Physiologiae Plantarum
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
22
Sayı
2
Künye
Babaoğlu, M., McCabe, M. S., Power, J. B., Davey, M. R., (2000). Agrobacterium-Mediated Transformation of Lupinus Mutabilis L. Using Shoot Apical Explants. Acta Physiologiae Plantarum, 22(2), 111-119. Doi: 10.1007/s11738-000-0064-8
