Agrobacterium-Mediated Transformation of Lupinus Mutabilis L. Using Shoot Apical Explants

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dc.contributor.authorBabaoğlu, Mehmet
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorPower, J. Brian
dc.contributor.authorDavey, Michael R.
dc.date.accessioned2020-03-26T16:36:25Z
dc.date.available2020-03-26T16:36:25Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA procedure for regenerating plants of Lupinus mutabilis from shoot apices, from which the leaf primordia and initial cell layer(s) of the apical meristem were removed, has been used to generate transgenic plants following Agrobacterium tumefaciens-mediated gene delivery. Transformation competent cells, from which buds developed, were located at the periphery of the apical meristem. Kanamycin resistant plants were obtained which expressed ?-glucuronidase activity. Integration of the neomycin phosphotransferase II and ?-glucuronidase genes into the genomes of transgenic plants was confirmed by nonradioactive DNA-DNA hybridisation. This is the first report of the generation of transgenic plants in L. mutabilis.en_US
dc.identifier.citationBabaoğlu, M., McCabe, M. S., Power, J. B., Davey, M. R., (2000). Agrobacterium-Mediated Transformation of Lupinus Mutabilis L. Using Shoot Apical Explants. Acta Physiologiae Plantarum, 22(2), 111-119. Doi: 10.1007/s11738-000-0064-8
dc.identifier.doi10.1007/s11738-000-0064-8en_US
dc.identifier.endpage119en_US
dc.identifier.issn0137-5881en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage111en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11738-000-0064-8
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17296
dc.identifier.volume22en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorBabaoğlu, Mehmet
dc.language.isoenen_US
dc.publisherPolish Academy of Sciencesen_US
dc.relation.ispartofActa Physiologiae Plantarumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAgrobacterium tumefaciensen_US
dc.subjectLupinus mutabilis L. (Lupin)en_US
dc.subjectShoot apicesen_US
dc.subjectTransformationen_US
dc.subjectTransgenic plantsen_US
dc.titleAgrobacterium-Mediated Transformation of Lupinus Mutabilis L. Using Shoot Apical Explantsen_US
dc.typeArticleen_US

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