PCR-SSCP-DNA Sequencing Method in Detecting PTEN Gene Mutation and Its Significance in Human Breast Cancer in Turkish Populations

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dc.contributor.authorVural Cingilli, Hasibe
dc.date.accessioned2020-03-26T18:31:00Z
dc.date.available2020-03-26T18:31:00Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to analyze the possible effect of PTEN gene mutations on the occurrence and development of human breast cancer. PCR-SSCP-DNA sequencing indicated that two kinds of mutation sites were found in 4 out of 80 breast cancer samples. One kind of mutation was found in exons. AA-TCC mutation was located 40 bp upstream of the 3'lateral exon 2 (115946 AA-TCC). Such mutations led to terminator formation at codon 267 of the PTEN gene. The other mutation was found in an intron, including a C-T point mutation 91 bp upstream of 2'lateral exon 2 (1903858 C-T). The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTEN protein expression was 68 % in breast cancer tissue, which was significantly lower than that (100 %) in paracancerous tissues (P < 0.005). PTEN gene mutation may play an important role in the occurrence and development of breast cancer.en_US
dc.description.sponsorshipSelcuk University Archeometry-Biotechnoogy LaboratorySelcuk University; Scientific Research Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipThe authors thank the members of Archeametry and Biotechnology, Konya, Turkey and the Department of Pathology in the Faculty of Medicine at the University of Cumhuriyet, Turkey, for their helpful comments and discussion. This study was partially supported by Selcuk University Archeometry-Biotechnoogy Laboratory and Scientific Research Foundation of Selcuk University (BAP).en_US
dc.identifier.citationCingilli Vural, H., (2012). PCR-SSCP-DNA Sequencing Method in Detecting PTEN Gene Mutation and Its Significance in Human Breast Cancer in Turkish Populations. Biotechnology & Biotechnological Equipment, 26(5), 3220-3223. DOI: 10.5504/BBEQ.2012.0084
dc.identifier.doi10.5504/BBEQ.2012.0084en_US
dc.identifier.endpage3223en_US
dc.identifier.issn1310-2818en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage3220en_US
dc.identifier.urihttps://dx.doi.org/10.5504/BBEQ.2012.0084
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28286
dc.identifier.volume26en_US
dc.identifier.wosWOS:000310398800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorVural Cingilli, Hasibe
dc.language.isoenen_US
dc.publisherDIAGNOSIS PRESS LTDen_US
dc.relation.ispartofBiotechnology & Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectbreast canceren_US
dc.subjectPTEN geneen_US
dc.subjectPCR-SSCPen_US
dc.subjectmutationen_US
dc.titlePCR-SSCP-DNA Sequencing Method in Detecting PTEN Gene Mutation and Its Significance in Human Breast Cancer in Turkish Populationsen_US
dc.typeArticleen_US

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