Analyzes of aflatoxin B1 and zearalenone in corn silage by high performance thin layer chromatography (HPTLC)-fluorodensitometric method

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dc.contributor.authorAydin, Hasan
dc.contributor.authorOguz, Halis
dc.date.accessioned2020-03-26T18:23:51Z
dc.date.available2020-03-26T18:23:51Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümüen_US
dc.description.abstractIn this study development of experimented and verified method available for routine analyzes of aflatoxin B-1 (AFB(1)) and zearalenone (ZON) contamination in corn silage by High Performance Thin Layer Chromatography (HPTLC)-Fluorodensitometric method; and determination of contamination in corn silage samples collected from all of the Central Anatolia provinces by this method were aimed. The validation of both methods used in AFB(1) and ZON analyses was performed by the parameters of specificity, linearity, recovery, sensitivity and precision. In the mycotoxin analyses, the limits of sensitivity were determined 2 ppb for AFB(1) and 12 ppb for ZON. The recovery was 74% AFB(1) and 81% for ZON in corn silage. Chloroform + methanol + water development solution was used for AFB(1) analyses; and HPTLC plates were used instead of TLC plates in ZON analyses. A total of 260 corn silage samples which were the crops of the year 2007 were collected in the dairy cattle and sheep enterprises in the provinces of Aksaray, Ankara, Cankiri, Eskisehir, Karaman, Kayseri, Kirikkale, Kirsehir, Konya, Nevsehir, Nigde, Sivas and Yozgat in Central Anatolia-Turkey between the dates of March 1 and May 31, 2008; and AFB(1) and ZON levels in collected corn silage samples were analyzed. The tested and verified methods whose effectiveness was experimented on the silage samples were determined in laboratories for routine analyses. The result of toxicological analyzes, AFB(1) and ZON contamination were not detected in any of the silage samples.en_US
dc.identifier.citationAydın, H., Oğuz, H. (2012). Analyzes of Aflatoxin B1 and Zearalenone in Corn Silage by High Performance Thin Layer Chromatography (HPTLC)-Fluorodensitometric Method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(1), 151-156.
dc.identifier.endpage156en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue1en_US
dc.identifier.startpage151en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27742
dc.identifier.volume18en_US
dc.identifier.wosWOS:000302228400027en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorOguz, Halis
dc.language.isotren_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCorn silageen_US
dc.subjectAflatoxin B-1en_US
dc.subjectZearalenoneen_US
dc.subjectHPTLCen_US
dc.subjectFlourodensitometryen_US
dc.titleAnalyzes of aflatoxin B1 and zearalenone in corn silage by high performance thin layer chromatography (HPTLC)-fluorodensitometric methoden_US
dc.title.alternativeMısır silajında aflatoksin B1 ve zearalenon kirliliklerinin yüksek performanslı ince tabaka kromatografisi (HPTLC)- florodansitometrik yöntemle belirlenmesien_US
dc.typeArticleen_US

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