Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

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dc.contributor.authorUgur M.R.
dc.contributor.authorDinh T.
dc.contributor.authorHitit M.
dc.contributor.authorKaya A.
dc.contributor.authorTopper E.
dc.contributor.authorDidion B.
dc.contributor.authorMemili E.
dc.date.accessioned2020-03-26T20:20:40Z
dc.date.available2020-03-26T20:20:40Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSperm cryopreservation is an important technique for fertility management, but post-thaw viability of sperm differs among breeding bulls. With metabolites being the end products of various metabolic pathways, the contributions of seminal plasma metabolites to sperm cryopreservation are still unknown. These gaps in the knowledge base are concerning because they prevent advances in the fundamental science of cryobiology and improvement of bull fertility. The objective of this study was to test the hypothesis that seminal plasma amino acids are associated with freezability of bull sperm. To accomplish this objective, amino acid concentrations in seminal plasma from seven bulls of good freezability (GF) and six bulls of poor freezability (PF) were quantified using gas chromatography–mass spectrometry (GC–MS). Multivariate and univariate analyses were performed to identify potential freezability biomarkers. Pathways and networks analyses of identified amino acids were performed using bioinformatic tools. By analyzing and interpreting the results we demonstrated that glutamic acid was the most abundant amino acid in bull seminal plasma with average concentration of 3,366 ± 547.3 nM, which accounts for about 53% of total amino acids. The other most predominant amino acids were alanine, glycine, and aspartic acid with the mean concentrations of 1,053 ± 187.9, 429.8 ± 57.94, and 427 ± 101.3 nM. Pearson’s correlation analysis suggested that phenylalanine concentration was significantly associated with post-thaw viability (r = 0.57, P-value = 0.043). Significant correlations were also found among other amino acids. In addition, partial least squares-discriminant analysis (PLS-DA) bi-plot indicated a distinct separation between GF and PF groups. Phenylalanine had the highest VIP score and was more abundant in the GF groups than in the PF groups. Moreover, pathway and network analysis indicated that phenylalanine contributes to oxidoreductase and antioxidant reactions. Although univariate analysis did not yield significant differences in amino acid concentration between the two groups, these findings are significant that they indicate the potentially important roles of amino acids in seminal plasma, thereby building a foundation for the fundamental science of cryobiology and reproductive biotechnology. © Copyright © 2020 Ugur, Dinh, Hitit, Kaya, Topper, Didion and Memili.en_US
dc.description.sponsorshipMississippi Agricultural and Forestry Experiment Station, Mississippi State University National Institute of Food and Agriculture Türkiye Bilimsel ve Teknolojik Araştirma Kurumuen_US
dc.description.sponsorshipFunding. This project was supported by the Agriculture and Food Research Initiative Competitive Grant No. 2017-67016-26507 from the USDA National Institute of Food and Agriculture. Partial funding was provided by the Mississippi Agricultural Forestry Experiment Station, and by Alta Genetics Inc., Watertown, WI, United States. MH was funded through a competitive international postdoctoral fellowship by the Scientific and Technological Research Council of Turkey (TUBITAK). MU was funded through a competitive graduate fellowship from the Turkish Ministry of National Education.en_US
dc.identifier.doi10.3389/fcell.2019.00347en_US
dc.identifier.issn2296634Xen_US
dc.identifier.pmid31993417en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://dx.doi.org/10.3389/fcell.2019.00347
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38642
dc.identifier.volume7en_US
dc.identifier.wosWOS:000550702800001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Cell and Developmental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectamino acidsen_US
dc.subjectbull spermen_US
dc.subjectfreezabilityen_US
dc.subjectmetabolomicsen_US
dc.subjectseminal plasmaen_US
dc.titleAmino Acids of Seminal Plasma Associated With Freezability of Bull Spermen_US
dc.typeArticleen_US

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