Plasma metabolomics in calves with acute bronchopneumonia

Basit Öğe Kaydı
dc.contributor.authorBasoglu, Abdullah
dc.contributor.authorBaspinar, Nuri
dc.contributor.authorTenori, Leonardo
dc.contributor.authorVignoli, Alessia
dc.contributor.authorYildiz, Ramazan
dc.date.accessioned2020-03-26T19:25:39Z
dc.date.available2020-03-26T19:25:39Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground Bovine respiratory disease is one of the main health issues in dairy calves. Inflammatory lung diseases are highly complex with respect to pathogenesis and relationships between inflammation, clinical disease and response to treatment. Metabolomics may offer the potential to identify biomarkers that define calf bronchopneumonia in terms of combined clinical, physiological and patho-biological abnormalities. While metabolomic studies are often encountered in childhood pneumonia, there is no knowledge related to the same approach to calf pneumonia. Objective The aim of this first study was to reveal the new potential biomarkers for acute calf bronchopneumonia by single proton (H-1) Nuclear magnetic resonance (NMR) based quantitative metabolomics. Methods Fifty dairy calves with acute bronchopneumonia presented for treatment to the teaching hospital, and ten healthy dairy calves belonging the teaching farm were used. Laboratory (hematological: complete blood count and blood gas analysis, and biochemical analysis related to health profile) were performed. NMR spectra of the all samples (50 diseased + 10 healthy water soluble extracts, 50 diseased + 10 healthy lipid extracts) were acquired using a standard Nuclear Overhauser Effect Spectroscopy pulse sequence. Results NMR based metabolomics analysis showed that calves suffering from bronchopneumonia and healthy calves have two different and distinguishable metabolic fingerprints using both water soluble and lipid extracts. Alterations in metabolites, increases in 2-methyl glutarate, phenylalanine, phosphatidylcholine, and decreases in ethanol, dimethylsulfone, propionate, acetate, allantoin, free cholesterol, cholesterol (-C18), were meaningful for pathogenic mechanisms of calf bronchopneumonia. Conclusion The NMR based metabolomics may contribute to better understanding bronchopneumonia in calves.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordination UnitSelcuk University [15401135]; EC iNEXT [653706]; Fondazione VeronesiFondazione Umberto Veronesien_US
dc.description.sponsorshipThis work was financially supported by Selcuk University Scientific Research Projects Coordination Unit (Project No: 15401135). CERM/CIRMMP center of the ESFRI Instruct is gratefully acknowledged for the NMR access provision financially supported by the EC Contract iNEXT No 653706. This work was partially supported by Fondazione Veronesi that granted L. T through the Post-Doctoral Fellowship-2015.en_US
dc.identifier.doi10.1007/s11306-016-1074-xen_US
dc.identifier.issn1573-3882en_US
dc.identifier.issn1573-3890en_US
dc.identifier.issue8en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11306-016-1074-x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33882
dc.identifier.volume12en_US
dc.identifier.wosWOS:000381083100002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofMETABOLOMICSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMetabobolomicsen_US
dc.subjectNMRen_US
dc.subjectCalfen_US
dc.subjectBronchopneumoniaen_US
dc.titlePlasma metabolomics in calves with acute bronchopneumoniaen_US
dc.typeArticleen_US

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