Stepwise rising CO2 insufflation as an ischemic preconditioning method

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dc.contributor.authorŞahin, Dursun A.
dc.contributor.authorHaliloğlu, Berna
dc.contributor.authorŞahin, Figen Kır
dc.contributor.authorAkbulut, Gökhan
dc.contributor.authorFidan, Hüseyin
dc.contributor.authorKöken, Gülengül
dc.contributor.authorBüyükbaş, Sadık
dc.date.accessioned2020-03-26T17:18:15Z
dc.date.available2020-03-26T17:18:15Z
dc.date.issued2007
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: The pneumoperitoneum (Pp) is associated with ischemia and reperfusion (I/ R) injury and oxidative stress. Various ischemic-preconditioning (IP) methods were used to reduce ischemic injury in intra-abdominal organs. In this experimental, randomized, controlled trial with a blind assessment of the outcome, we evaluated the effects of a new IP method, stepwise rising CO2 insufflation, on oxidative stress and inflammatory cytokine response. Methods: Twenty-one rats were divided into three groups. Rats in the control group were subjected to general anesthesia for only 60 minutes. The stepwise group was subjected to 5 mm Hg for 10 minutes, 10 mm Hg for 10 minutes, and 15 mm Hg of CO2 insufflation for 60 minutes without deflation. In the Pp15 group, the pressure of CO2 insufflation was fixed at 15 mm Hg for 60 minutes without deflation. Liver and blood samples were examined to determine malondialdehyde (MDA), the antioxidant, superoxide dismutase (SOD), and inflammatory cytokine (tumor necrosis factor-alpha [TNF-alpha], interleukin-6 [IL-6]) levels. Histopathologic scores of liver tissue were examined in all groups. Results: The highest plasma and liver MDA, TNF-alpha, and IL-6 values were in the Pp15 group, followed by the stepwise and control groups. However, plasma and liver SOD levels determined in the control group were significantly higher, compared to stepwise and Pp15 groups. The lowest plasma and liver levels of SOD were in the Pp15 group, followed by the stepwise and control groups. Significantly higher histopathologic scores were found in the Pp15 group, followed by the stepwise and control groups, as well as MDA and inflammatory cytokine (TNF-alpha, IL-6) levels. Conclusions: We concluded that the stepwise rising CO2 insufflation method may be an alternative IP method that may lead to a reduction in I/ R injury.en_US
dc.identifier.doi10.1089/lap.2007.0008en_US
dc.identifier.endpage729en_US
dc.identifier.issn1092-6429en_US
dc.identifier.issn1557-9034en_US
dc.identifier.issue6en_US
dc.identifier.pmid18158800en_US
dc.identifier.startpage723en_US
dc.identifier.urihttps://dx.doi.org/10.1089/lap.2007.0008
dc.identifier.urihttps://hdl.handle.net/20.500.12395/21621
dc.identifier.volume17en_US
dc.identifier.wosWOS:000252186800004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMARY ANN LIEBERT, INCen_US
dc.relation.ispartofJOURNAL OF LAPAROENDOSCOPIC & ADVANCED SURGICAL TECHNIQUESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleStepwise rising CO2 insufflation as an ischemic preconditioning methoden_US
dc.typeArticleen_US

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