Medicago rigidula (l.) all.’nın antioksidan ve enzim inhibisyon aktiviteleri ve fenolik bileşiminin incelenmesi
Yükleniyor...
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MARMARA UNIV, FAC PHARMACY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Çalışma Medicago rigidula (L.) All. metanol ekstresinin antioksidan kapasitesi, fenolik bileşimi ve enzim inhibisyon aktivitesinin değerlendirilmesi üzerine odaklanmıştır. Antioksidan aktivite 2,2-difenill-1-pikrilhidrazil (DPPH), demir indirgeme antioksidan gücü (FRAP), bakır iyonu indirgeme gücü (CUPRAC) ve total antioksidan kapasite (fosfomolibdat) testini içeren farklı yöntemler kullanılarak değerlendirilmiştir. Aynı zamanda total fenolik ve flavonoit içerikler de belirlenmiştir. Enzim inhibisyon aktiviteleri antikolinesteraz, tirozinaz, alfa-amilaz ve alfa-glukozidaza karşı incelenmiştir. M. rigidula bitkisinin fenolik bileşenlerinin kateşin, sinnamik asit, klorojenik asit, gallik asit ve sirinjik asit bileşikleri olduğu saptanmıştır. Metanol ekstresinin serbest radikal giderici aktivitesi 1 mg/mL konsantrasyonda %20.30’dur. Fosfomolibdat ve FRAP testlerinde sırası ile 167.27 mg AAE/g ve 67.01 mg TE/g bulundu. Total fenolik ve flavonoit içerik sırasıyla 79.61 mg GAE/g ve 27.38 µg QE/g olarak belirlenmiştir. Sonuçlar M. rigidula’nın iyi bir antioksidan ve enzim inhibisyon kapasitesine sahip olduğunu gösterdi. Bu nedenle Medicago rigidula bitkisinin gıda ve ilaç endüstrisinde hammadde olarak kullanılabileceği düşünülmektedir.
The study focused on evaluating of antioxidant capacity, phenolic compounds and enzyme inhibition activity of methanolic extract Medicago rigidula. Antioxidant activity was evaluated using different assays including 2,2-diphenyl-1-pikrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), cupric ion reducing antioxidant capacity (CUPRAC) and total antioxidant capacity (phosphomolybdenum) test. Total phenolic and flavonoit contents were also determined. Enzyme inhibition activities were examined against anticholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. Phenolic content of M. rigidula included catechin, cinnamic, chlorogenic, gallic and syringic acids. Free radical scavenging activity of methanol extract was 20.30% at 1 mg/mL concentration. In phosphomolybdenum and FRAP tests were found 167.27 mg AAE/g and 67.01 mg TE/g, respectively. Total phenolic and flavonoid contents were determined as 79.61 mg GAE/g and 27.38 mu g QE/g, respectively. The results showed that M. rigidula has a good source of antioxidants and enzyme inhibition capacity. Thus, it can be used as raw material in food and pharmaceutical industry.
The study focused on evaluating of antioxidant capacity, phenolic compounds and enzyme inhibition activity of methanolic extract Medicago rigidula. Antioxidant activity was evaluated using different assays including 2,2-diphenyl-1-pikrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), cupric ion reducing antioxidant capacity (CUPRAC) and total antioxidant capacity (phosphomolybdenum) test. Total phenolic and flavonoit contents were also determined. Enzyme inhibition activities were examined against anticholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. Phenolic content of M. rigidula included catechin, cinnamic, chlorogenic, gallic and syringic acids. Free radical scavenging activity of methanol extract was 20.30% at 1 mg/mL concentration. In phosphomolybdenum and FRAP tests were found 167.27 mg AAE/g and 67.01 mg TE/g, respectively. Total phenolic and flavonoid contents were determined as 79.61 mg GAE/g and 27.38 mu g QE/g, respectively. The results showed that M. rigidula has a good source of antioxidants and enzyme inhibition capacity. Thus, it can be used as raw material in food and pharmaceutical industry.
Açıklama
Anahtar Kelimeler
Kimyasal kompozisyon, Biyoaktivite, HPLC analizi, Chemical composition, Bioactivity, HPLC analysis, Fabaceae
Kaynak
Marmara Pharmaceutical Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
21
Sayı
3
Künye
Çakmak, Y. S., Zengin, G., Eskin, B., Yıldırım, K., Topal, M., Aydın, G. H., Ünlü, E. Baydemir, M., Erten, K. (2017). Medicago rigidula (L.) ALL.’nın Antioksidan ve Enzim İnhibisyon Aktiviteleri ve Fenolik Bileşiminin İncelenmesi. Marmara Pharmaceutical Journal, 21(3), 522-529.
