The effect of 12 successive blood passages on the virulence of Babesia ovis in splenectomized lambs: A preliminary study

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dc.contributor.authorSevinc, Ferda
dc.contributor.authorSevinc, Mutlu
dc.contributor.authorKoc, Yilmaz
dc.contributor.authorAlkan, Fahrettin
dc.contributor.authorEkici, Ozlem Derinbay
dc.contributor.authorYildiz, Ramazan
dc.contributor.authorIsik, Nermin
dc.date.accessioned2020-03-26T18:58:35Z
dc.date.available2020-03-26T18:58:35Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBabesia ovis, the primary causative agent of ovine babesiosis, is a tick-borne hemoprotozoan parasite and causes serious losses in small ruminant livestock in tropical and subtropical regions. The current control of ovine babesiosis has been base on chemotherapy and limited tick control measures. Attenuated parasites which are valuable sources of live vaccines have been used to prevent babesiosis in cattle. There is a necessity to the studies about prevention of the disease by means of immunoprophylaxis in sheep. For this purpose, this study was planned to obtain the attenuated derivatives of a virulent B. ovis strain by successive blood passages in splenectomized lambs. A total of seventeen, six-month-old lambs, 13 of which splenectomized, 4 of which spleen intact lambs, known to be negative for the presence of hemoparasites and anti-B. ovis antibodies were used. The splenectomized lambs were used for passaging the virulent B. ovis field strain. Four intact lambs divided into two groups were used to evaluate the pathogenicity of the virulent strain and twelfth passage strain of B. ovis. Blood collected from a clinically evident case of babesiosis in the field was inoculated intravenously into the first splenectomized lamb, and then the acute clinical disease was occurred. The same process was successively applied to the rest of 12 splenectomized lambs to have the attenuated B. ovis strain. The clinical, microscopic and hematological changes after inoculation were recorded in each passage of the lambs. All of the passage lambs were exhibited the severe symptoms of the disease from the 2nd day of inoculation. The same treatment protocol including vitamin B12 and the anti-babesial drug imidocarb dipropionate (IMDP) was applied to all splenectomized lambs on the fourth or fifth day of the inoculation. Although the symptoms of the disease were less severe than those of splenectomized lambs; two intact lambs infected with the virulent strain and two intact lambs infected with the twelfth passage blood were also showed the clinical and hematological findings of the disease on the 2nd and 3rd day after inoculation. Infection-related symptoms in all intact lambs disappeared spontaneously from the 6th day of inoculation without the application of any drug. The results of the study showed that there was no change on the virulence of B. ovis through the 12 successive blood passages. Therefore, the further passages or alternative attenuation methods may be necessary to attenuate the virulent strain of B. ovis. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipBAP (The Coordination of Scientific Research Projects, University of Selcuk)Gaziosmanpasa University [10401122]en_US
dc.description.sponsorshipThe authors would like to thank BAP (The Coordination of Scientific Research Projects, University of Selcuk, No. of Project: 10401122) for financial support.en_US
dc.identifier.doi10.1016/j.smallrumres.2013.10.010en_US
dc.identifier.endpage70en_US
dc.identifier.issn0921-4488en_US
dc.identifier.issn1879-0941en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage66en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.smallrumres.2013.10.010
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31184
dc.identifier.volume116en_US
dc.identifier.wosWOS:000329147800010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofSMALL RUMINANT RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectOvine babesiosisen_US
dc.subjectBabesia ovisen_US
dc.subjectSuccessive blood passagesen_US
dc.titleThe effect of 12 successive blood passages on the virulence of Babesia ovis in splenectomized lambs: A preliminary studyen_US
dc.typeArticleen_US

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