Diagnosis of feline infectious peritonitis by immunohistochemistry and histopathology methods: A case report based on diagnostic approach
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz Bu çalışmada, 6 aylık, tekir bir erkek kedide histopatolojik ve immünohistokimyasal (IHC) yöntemlerle kedi enfeksiyöz peritonit (FIP) vakası, tanımlanmıştır. Yapılan nekropside, karın boşluğunda önemli miktarda sarı renkli bir sıvı birikimi ile karaciğer, bağırsak serozası ve peritonda granüler tarzda fibrinöz bir eksüdat görüldü. Mikroskobik olarak bağırsakların tunika serosa tabakasındaki küçük ve orta çaplı damarların etrafında yoğun olarak makrofaj, lenfosit ve plazma hücrelerinden oluşan yangısel hücre infiltrasyonu ve fibrin eksüdasyonu belirlendi. Karaciğerde ise paranşime yayılmış, çeşitli boyutlarda nekrozlu veya nekrozsuz birçok granülomatöz odak bulundu. Methyl green pyronin boyamasında, plazma hücrelerinin, lezyonlarda bulunan inflamatuar hücrelerin çoğunluğunu oluşturduğu tespit edildi. Etkene karşı spesifik antikorlarla yapılan IHC boyamasında, bağırsakların serosa tabakasındaki granülomatöz lezyonlarda ve daha az sıklıkla lamina propriadaki mononükleer hücrelerin sitoplazmasında immünopozitivite elde edildi. Karaciğerde ise hem granülom hem de piyogranülomların etrafındaki makrofajların sitoplazmasında immünoreaktivite belirlendi. Tüm bulgular birlikte değerlendirildiğinde bu FIP olgusunun kuru form olarak ba
Aim: In this study the case of feline infectious peritonitis (FIP) has been identified as histopathological and immunohistochemical (IHC) in a 6-month-old male domestic shorthair cat. In necropsy, the abdominal cavity contained a significant volume of yellow liquid and granular fibrinous exudate was seen on the liver, serosa of intestines, and peritoneum. Microscopically, inflammatory cell infiltration and fibrin exudation, consisting mainly of macrophage, lymphocyte and plasma cells, were determined around small and medium-sized vessels in the tunica serosa layer of the intestines. Many granulomatous foci of various sizes, with or without necrosis, were found in the liver, spread to the parenchyma. In methyl green pyronin staining, plasma cells were found to be the majority of the inflammatory cells present in lesions. In IHC staining with specific antibodies against the agent, immunopositivity was obtained in granulomatous lesions in the serosa layer of the intestines and less frequently in the cytoplasm of mononuclear cells in the lamina propria. Immunoreactivity was detected in the cytoplasm of macrophages in the liver, around both pyrogranulomas and granulomas. It was concluded that this case of FIP, when all findings are evaluated together, may have started as a dry form, and turned into a wet form in the terminal period.
Aim: In this study the case of feline infectious peritonitis (FIP) has been identified as histopathological and immunohistochemical (IHC) in a 6-month-old male domestic shorthair cat. In necropsy, the abdominal cavity contained a significant volume of yellow liquid and granular fibrinous exudate was seen on the liver, serosa of intestines, and peritoneum. Microscopically, inflammatory cell infiltration and fibrin exudation, consisting mainly of macrophage, lymphocyte and plasma cells, were determined around small and medium-sized vessels in the tunica serosa layer of the intestines. Many granulomatous foci of various sizes, with or without necrosis, were found in the liver, spread to the parenchyma. In methyl green pyronin staining, plasma cells were found to be the majority of the inflammatory cells present in lesions. In IHC staining with specific antibodies against the agent, immunopositivity was obtained in granulomatous lesions in the serosa layer of the intestines and less frequently in the cytoplasm of mononuclear cells in the lamina propria. Immunoreactivity was detected in the cytoplasm of macrophages in the liver, around both pyrogranulomas and granulomas. It was concluded that this case of FIP, when all findings are evaluated together, may have started as a dry form, and turned into a wet form in the terminal period.
Açıklama
Anahtar Kelimeler
Koronavirüs, peritonit, kuru FİP, piyogranülom, kedi, Coronavirus, peritonitis, dry FIP, pyogranuloma, feline
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
37
Sayı
1
Künye
Ateş, M. B., Akçakavak, G., Çelik, Z., Özdemir, Ö., (2021). Diagnosis of feline infectious peritonitis by immunohistochemistry and histopathology methods: A case report based on diagnostic approach. Eurasian Journal of Veterinary Sciences, 37 (1), 55-61.
DOI: 10.15312/EurasianJVetSci.2021.326
