İki farklı rezin modifiye cam iyonomer simanın bölgesel toksisitesinin değerlendirilmesi
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı iki farklı rezin modifiye cam iyonomer simanın bölgesel toksisitesini değerlendirmektir. Gereç ve Yöntemler: Fuji II LC Capsule (GC) ve Vitrebond (3M ESPE) örnekleri üreticilerinin talimatlarına göre standart teflon disklerde hazırlandı. Örnekler, örnek/solüsyon hacmi 91,6mm2 /ml olacak şekilde 24 saat kültür ortamında bekletildi. L929 hücreleri 96 kuyucuklu hücre kültür kaplarına alındı ve 24 saat 37C°'de %10 FBS ve %1 penisilin/streptomisin içeren DMEM (Dulbecco's Modified Eagle's Medium) kültür ortamında bekletildi. Elde edilen materyallerin seyreltilmemiş ekstraktı ve 1/2, 1/4, 1/8, 1/16, 1/32 oranında seyreltilmiş olan ekstraktları hücrelere uygulandı. Hücre canlılığı 24. saatin sonunda XTT (2,3- Bis(2-metoksi-4-nitro-5-sulfofenil)-2H-tetrazolyum) testi ile belirlendi. Kontrol grubunun canlılığı %100 olacak şekilde kabul edildi ve tüm grupların canlılık yüzdesi buna göre belirlendi (n=27). İstatistiksel değerlendirmeler için one way ANOVA ve post hoc Tukey's HSD testleri kullanıldı. Her bir materyalin L929 hücrelerinin canlılıklarına ve proliferasyonlarına nasıl etki ettiği gerçek zamanlı hücre analizi yöntemi ile 15 dakikada bir empedans ölçümü alınarak izlendi. Elde edilen verilerin analizi RTCA Software 2.0 programı ile gerçekleştirildi, istatistiksel olarak hiyerarşik kümeleme analizi yapıldı. Bulgular: XTT deneyi sonucunda Fuji II LC’nin seyreltilmemiş konsantrasyonu, Vitrebond’un ise seyreltilmemiş, 1/2 ve 1/4 oranında seyreltilmiş konsantrasyonlarının L929 fibroblast hücreleri üzerine sitotoksik etkileri olduğu izlendi (p<0,05). Gerçek zamanlı hücre analiz deneyi sonuçlarına göre Fuji II LC Capsule’ün seyreltilmemiş konsantrasyon grubunda, Vitrebond’un ise seyreltilmemiş, 1/2, 1/4 ve 1/8 konsantrasyon gruplarında hücre canlılığının 144. saat sonunda tamamen kaybolduğu görülmüştür. Sonuç: Rezin modifiye cam iyonomer simanların biyolojik olarak aktif içerikleri pulpa hücrelerinin metabolizmasını değiştirebilecek sitotoksik potansiyele sahip olabilir. Bu nedenle özellikle derin kavitelerde materyal seçimine dikkat edilmelidir.
Background: The aim of this study was to evaluate the regional toxicity of two different resin modified glass ionomer cement. Methods: Fuji II LC Capsule (GC) and Vitrebond (3M ESPE) material specimens were prepared according to manufacturers’ instructions with using standard teflon matrix (2x5mm). Samples were kept in culture medium for 24 hours with a sample surface area/solution volume ratio of 91.6 mm2 / ml. L929 cells were taken into 96-well plates and incubated for 24 hours at 37°C in DMEM (Dulbecco's Modified Eagle's Medium) culture medium with containing 10% FBS and 1% penicillin/streptomycin. The acquired material undiluted extract and 1/2, 1/4, 1/8, 1/16, 1/32 of the diluted extracts were applied to the cells. In the control group, only serum-containing culture medium was added to the cells. Cell viability determined by XTT (2,3-Bis (2-methoxy-4- nitro-5-sulfophenyl) -2H-tetrazolium) test at the end of 24 hours. The viability of the control group was accepted to be 100% and the viability of all other groups was determined accordingly. ANOVA and post hoc Tukey's HSD tests were used for statistical evaluations. The effect of each material on the viability and proliferation of L929 cells was monitored by real-time cell analysis method by taking impedance measurement every 15 minutes. The analysis of the obtained data was performed by RTCA Software 2.0 program, thereafter statistically analyzed by hierarchical clustering method. Results: XTT test showed that undiluted concentration of Fuji II LC and Vitrebond's undiluted, 1/2 and 1/4 diluted concentrations had cytotoxic effects on L929 fibroblast cells (p <0.05). In real time cell analysis Fuji II LC Capsule’s undiluted concentration group and Vitrebond's undiluted, 1/2, 1/4, 1/8 concentration groups cell viability completely disappeared after 144 hours. Conclusion: Biologically active ingredients of resin modified glass ionomer cements may have cytotoxic potential that may alter the metabolism of pulp cells. Therefore, especially in deep cavities material selection should be careful.
Background: The aim of this study was to evaluate the regional toxicity of two different resin modified glass ionomer cement. Methods: Fuji II LC Capsule (GC) and Vitrebond (3M ESPE) material specimens were prepared according to manufacturers’ instructions with using standard teflon matrix (2x5mm). Samples were kept in culture medium for 24 hours with a sample surface area/solution volume ratio of 91.6 mm2 / ml. L929 cells were taken into 96-well plates and incubated for 24 hours at 37°C in DMEM (Dulbecco's Modified Eagle's Medium) culture medium with containing 10% FBS and 1% penicillin/streptomycin. The acquired material undiluted extract and 1/2, 1/4, 1/8, 1/16, 1/32 of the diluted extracts were applied to the cells. In the control group, only serum-containing culture medium was added to the cells. Cell viability determined by XTT (2,3-Bis (2-methoxy-4- nitro-5-sulfophenyl) -2H-tetrazolium) test at the end of 24 hours. The viability of the control group was accepted to be 100% and the viability of all other groups was determined accordingly. ANOVA and post hoc Tukey's HSD tests were used for statistical evaluations. The effect of each material on the viability and proliferation of L929 cells was monitored by real-time cell analysis method by taking impedance measurement every 15 minutes. The analysis of the obtained data was performed by RTCA Software 2.0 program, thereafter statistically analyzed by hierarchical clustering method. Results: XTT test showed that undiluted concentration of Fuji II LC and Vitrebond's undiluted, 1/2 and 1/4 diluted concentrations had cytotoxic effects on L929 fibroblast cells (p <0.05). In real time cell analysis Fuji II LC Capsule’s undiluted concentration group and Vitrebond's undiluted, 1/2, 1/4, 1/8 concentration groups cell viability completely disappeared after 144 hours. Conclusion: Biologically active ingredients of resin modified glass ionomer cements may have cytotoxic potential that may alter the metabolism of pulp cells. Therefore, especially in deep cavities material selection should be careful.
Açıklama
Anahtar Kelimeler
Biyouyumluluk, sitotoksisite, XTT testi, rezin modifiye cam iyonomer simanlar, Biocompatibility, cytotoxicity, XTT assay, resin modified glass ionomer cements
Kaynak
Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
3
Künye
Kölüş, T., Ülker, H. E., (2020). İki farklı rezin modifiye cam iyonomer simanın bölgesel toksisitesinin değerlendirilmesi. Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 7 (3), 413-421. Doi: 10.15311/selcukdentj. 676906
