Effcacy of experimental inactivated and live Rhodococcus equi vaccines for thoroughbred Arabian mares in mice
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to determine the effcacy of inactive Rhodococcus equi vaccine candidates included that bacterinaluminum hydroxide (Al(OH)3), bacterinVapA(Al(OH)3), bacterinMontanide IMS 3012 (IMS), bacterinVapAIMS, and live vaccine using mice as a model. Te efcacy of vaccine was evaluated according to clinical fndings, humoral and cellular immunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrifced mice. Inactive R. equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injected once. Fifeen days afer the last vaccination, aerosol challenges were carried out with the pathogenic R. equi VapAK2002 strain in all groups. Two mice were sacrifced from each challenge groups on days 1, 3, 5, and 7. The antibody titers of vaccinated mice were found to be signifcantly higher than those of the controls. The largest number of INF-g positive samples were detected in the bacterinVapAIMS and bacterinIMS groups. IL-4 positivity was determined only in live vaccine groups. The lowest reisolation rate of R. equi from internal organs was observed in the bacterinVapAIMS group. It was concluded that R. equi vaccines, and especially bacterinVapAIMS, are useful to protect mice against R. equi infection.
Açıklama
Anahtar Kelimeler
Ziraat Mühendisliği
Kaynak
Turkish Journal of Veterinary and Animal Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
3
