The Investigation of Relationship Between Na+/K+ ATPase Enzyme Activity, ATP1A1 Gene Polymorphism and C-Peptide in Type 2 Diabetic Patients with Neuropathy
Küçük Resim Yok
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TURKISH BIOCHEM SOC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objectives: Na+/K+-ATPase is encoded by various genes, of which the ATP1A1 gene is expressed predominantly in peripheral nerves and in erythrocytes. We investigated whether the ATP1A1 polymorphism is associated with Na+/K+-ATPase activity modifications in healthy subjects and in type 2 diabetic patients with polyneuropathy. We also tested whether C-peptide level could influence the relationship between ATP1A1 polymorphism and Na+/K+-ATPase activity. Methods: Na+/K+-ATPase polymorphism has been determined by using polymerase-chain reactions and restriction fragment length polymorphism methods. The erythrocyte membrane Na+/K+-ATPase activity measurement was performed with modified Kitao-Hattori method. C-peptide measurements were performed using competitive chemiluminesence enzyme immunoassay method. Results: Na+/K+-ATPase activity was significantly lower in diabetic polyneuropathic patients than in healthy subjects. Correlation between C-peptide levels and Na+/K+-ATPase activity was not detected. All diabetic patients with polyneuropathy and healthy controls were homozygous for the unrestricted allele. Conclusion: Oxidative stress which develops in diabetes causes lipid peroxidation in the membrane and consequently Na+/K+-ATPase activity decreases. Low enzymatic activity leads to decrease in nerve conduction velocity. Therefore, we believe that better regulation of diabetes can abrogate complications and prevent the development of neuropathy.
Açıklama
Anahtar Kelimeler
Na+/K+-ATPase, Polymorphism, C-peptide
Kaynak
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
34
Sayı
3
