The Investigation of Relationship Between Na+/K+ ATPase Enzyme Activity, ATP1A1 Gene Polymorphism and C-Peptide in Type 2 Diabetic Patients with Neuropathy
dc.contributor.author | Topcu, Cemile | |
dc.contributor.author | Gürbilek, Mehmet | |
dc.contributor.author | Aköz, Mehmet | |
dc.contributor.author | Çora, Tülin | |
dc.date.accessioned | 2020-03-26T17:40:46Z | |
dc.date.available | 2020-03-26T17:40:46Z | |
dc.date.issued | 2009 | |
dc.department | Selçuk Üniversitesi | en_US |
dc.description.abstract | Objectives: Na+/K+-ATPase is encoded by various genes, of which the ATP1A1 gene is expressed predominantly in peripheral nerves and in erythrocytes. We investigated whether the ATP1A1 polymorphism is associated with Na+/K+-ATPase activity modifications in healthy subjects and in type 2 diabetic patients with polyneuropathy. We also tested whether C-peptide level could influence the relationship between ATP1A1 polymorphism and Na+/K+-ATPase activity. Methods: Na+/K+-ATPase polymorphism has been determined by using polymerase-chain reactions and restriction fragment length polymorphism methods. The erythrocyte membrane Na+/K+-ATPase activity measurement was performed with modified Kitao-Hattori method. C-peptide measurements were performed using competitive chemiluminesence enzyme immunoassay method. Results: Na+/K+-ATPase activity was significantly lower in diabetic polyneuropathic patients than in healthy subjects. Correlation between C-peptide levels and Na+/K+-ATPase activity was not detected. All diabetic patients with polyneuropathy and healthy controls were homozygous for the unrestricted allele. Conclusion: Oxidative stress which develops in diabetes causes lipid peroxidation in the membrane and consequently Na+/K+-ATPase activity decreases. Low enzymatic activity leads to decrease in nerve conduction velocity. Therefore, we believe that better regulation of diabetes can abrogate complications and prevent the development of neuropathy. | en_US |
dc.identifier.endpage | 159 | en_US |
dc.identifier.issn | 0250-4685 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 154 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12395/23956 | |
dc.identifier.volume | 34 | en_US |
dc.identifier.wos | WOS:000270935300007 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.language.iso | tr | en_US |
dc.publisher | TURKISH BIOCHEM SOC | en_US |
dc.relation.ispartof | TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.selcuk | 20240510_oaig | en_US |
dc.subject | Na+/K+-ATPase | en_US |
dc.subject | Polymorphism | en_US |
dc.subject | C-peptide | en_US |
dc.title | The Investigation of Relationship Between Na+/K+ ATPase Enzyme Activity, ATP1A1 Gene Polymorphism and C-Peptide in Type 2 Diabetic Patients with Neuropathy | en_US |
dc.type | Article | en_US |