Interaction of nucleic acid bases and nucleosides with cobalt ions immobilized in a column system

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dc.contributor.authorAyar, A
dc.contributor.authorMercimek, B
dc.date.accessioned2020-03-26T17:03:46Z
dc.date.available2020-03-26T17:03:46Z
dc.date.issued2006
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe interactions of ligands, such as adenine, adenosine, uracil and uridine with Co(II) ions immobilized by carboxylated diaminoethyl matrix on sporopollenin were investigated as a function of temperature by the breakthrough technique. The adsorption characteristics of ligands on Co(II)carboxylated diaminoethyl sporopollenin [Co(II)-CDAE-S] were investigated by Scatchard plot analysis under optimized conditions of flow rate and temperature. Equilibrium binding data used in Scatchard plot analysis for adenosine gave rise to a linear plot, indicating the presence of specific interaction and Langmuirean behavior. However, the adsorption of uracil on this ligand-exchange stationary phase was affected by the electrostatic or hydrophobic interaction in the whole concentration range. In the case of adenine and uridine, the adsorption performance was strongly affected by the initial ligand concentration. At high concentration, adenine and uridine were adsorbed specifically to the ligand exchanger matrix exposed on the adsorbent. At lower concentrations, the binding to the second sets of adsorbent was appeared because of nonspecific interaction such as hydrophobic and electrostatic. (c) 2006 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.procbio.2006.02.016en_US
dc.identifier.endpage1559en_US
dc.identifier.issn1359-5113en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1553en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.procbio.2006.02.016
dc.identifier.urihttps://hdl.handle.net/20.500.12395/20549
dc.identifier.volume41en_US
dc.identifier.wosWOS:000238237500012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCI LTDen_US
dc.relation.ispartofPROCESS BIOCHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectligand bindingen_US
dc.subjectbreakthrough curveen_US
dc.subjectscatchard plot analysisen_US
dc.subjectsporopolleninen_US
dc.titleInteraction of nucleic acid bases and nucleosides with cobalt ions immobilized in a column systemen_US
dc.typeArticleen_US

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