Evaluation of h beta D-1 and h beta D-2 Levels in Saliva of Patients with Oral Mucosal Diseases

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dc.contributor.authorKucukkolbasi, H.
dc.contributor.authorKucukkolbasi, S.
dc.contributor.authorAyyildiz, H. F.
dc.contributor.authorDursun, R.
dc.contributor.authorKara, H.
dc.date.accessioned2020-03-26T18:41:51Z
dc.date.available2020-03-26T18:41:51Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective: This study aimed to determine a possible correlation between oral mucosal disease and salivary concentrations of the antimicrobial peptides human beta-defensin-1 (h beta D-1) and human beta-defensin-2 (h beta D-2). Method: The present work focussed on the establishment of a reversed phase-high performance liquid chromatography (RP-HPLC) procedure to quantify human beta-defensins (h beta D-1 and h beta D-2) in saliva samples of patients with oral diseases such as lichen planus (n = 10), Behcet (n = 10) and recurrent apthous stomatitis (n = 10). Results: Linear calibration range for h beta D-1 and h beta D-2 defensins was 1.67-200 mu g mL(-1) and 3.13 - 100 mu g mL(-1) with R-2 values of 0.9998 and 0.996, correspondingly. The concentration of beta-defensins in saliva was determined by comparing the peak areas of eluted h beta D-1 and h beta D-2 with that of their standards. The variation of the amount of beta-defensins was evaluated by comparisons of the results obtained from the patients with oral mucosal diseases before and after treatments and the control subjects. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.62 mu g mL(-1) and 5.39 mu g mL(-1) for h beta D-1 and 0.94 mu g mL(-1) and 3.13 mu g mL(-1) for h beta D-2, respectively. Conclusion: The salivary beta-defensin concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients with oral mucosal diseases, the concentration was significantly higher before treatment than after treatment.en_US
dc.identifier.endpage238en_US
dc.identifier.issn0043-3144en_US
dc.identifier.issue3en_US
dc.identifier.pmid24564045en_US
dc.identifier.startpage230en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29485
dc.identifier.volume62en_US
dc.identifier.wosWOS:000329085600013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherUNIV WEST INDIES FACULTY MEDICAL SCIENCESen_US
dc.relation.ispartofWEST INDIAN MEDICAL JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDefensinen_US
dc.subjecth beta D-1en_US
dc.subjecth beta D-2en_US
dc.subjecthigh-performance liquid chromatography (HPLC)en_US
dc.subjectoral diseaseen_US
dc.subjectsalivaen_US
dc.titleEvaluation of h beta D-1 and h beta D-2 Levels in Saliva of Patients with Oral Mucosal Diseasesen_US
dc.typeArticleen_US

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