The effect of interferon alpha administration on cytokine levels in sheep
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Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın temel amacı, sağlıklı koyunlara parenteral rekombinant insan interferon (rHuIFN)-?2a uygulamasının serum tümör nekroz faktör (TNF)-?, interlöykin (IL)-6 ve IL-10 seviyeleri üzerindeki etkisini belirlemektir. Ayrıca sağlıklı koyunlarda temel fizyolojik parametreler (vücut ısısı, nabız, solunum hızı), hemogram ve kan gazı parametrelerine etkisini tespit etmektir. Gereç ve Yöntem: Bu çalışmada 10 Merinos koyuna 9.000.000 IU rHuIFN-?2a deri altı yolla uygulandı. Kan örnekleri uygulamadan önce (0 saat) ve sonra 4, 8, 12, 24, 48, 72, 96 ve 120 saatlerde alındı. Aynı örnekleme zamanlarında vücut ısısı, nabız ve solunum hızı da belirlendi. Serum örneklerinden koyun spesifik TNF-?, IL-6 ve IL-10 seviyeleri ELISA okuyucu ile ölçüldü. Hemogram ve kan gazı parametreleri sırasıyla tam kan hücresi sayım cihazı ve kan gazı analiz cihazında ölçüldü. Bulgular: rHuIFN-?2a uygulaması sonrasında TNF-? ve IL-10 konsantrasyonları 96. saatte pik düzeye (p<0.05) ulaşırken, IL-6 konsantrasyonunda istatistiksel olarak anlamlı değişim belirlenmedi (p>0.05). Ayrıca vücut ısısı ve pO2 düzeylerinde geçici artışlar (p<0.05) belirlenirken, potasyum ve iyonize kalsiyum seviyelerinde düşmeler (p<0.05) belirlendi. Nabız, akyuvar sayımı, pH, base(ecf) ve sodyum değerlerinde ise istatistiki dalgalanmalar (p<0.05) tespit edildi. Öneri: Koyunlara rHuIFN-?2a uygulamasının immünolojik etkiler gösterebileceği, genel olarak güvenli kabul edilebileceği ve tedavide kullanım için düşünülebileceği ifade edilebilir.
Aim: The primary aim of this study was to determine the effect of parenteral recombinant human interferon (rHuIFN)-?2a administration on serum tumor necrosis factor (TNF)-?, interleukin (IL)-6 and IL-10 levels in healthy sheep. In addition, the main physiological parameters (rectal temperature, pulse rate, respiratory rate), hemogram and blood gas parameters were determined, as well. Materials and Methods: In this study, 9.000.000 IU rHuIFN-?2a was administered subcutaneously in 10 Merinos sheep. Blood samples were taken before (0 hours) and after at 4, 8, 12, 24, 48, 72, 96 and 120 hours. Rectal temperature, pulse and respiratory rate were also determined at the same sampling times. Sheep-specific TNF-?, IL-6 and IL-10 levels were determined from serum samples by the ELISA reader. Hemogram and blood gas parameters were measured by complete blood cell counter and blood gas analyzer, respectively. Results: After rHuIFN-?2a treatment, TNF-? and IL-10 concentrations reached peak levels (p<0.05) at the 96 hours, whereas the IL-6 level did not change in a statistically significant (p>0.05). On the other hand, a temporary increase in rectal temperature and pO2 levels (p<0.05) were determined, while decreased potassium and ionized calcium levels (p<0.05) were measured. Statistically significantly (p<0.05) fluctuations were determined in pulse rate, white blood cell counts, pH, base(ecf) and sodium values. Conclusion: It may be stated that administration of rHuIFN-?2a showed immunological effects in sheep, as it is generally accepted as safe and can be considered for use in treatment.
Aim: The primary aim of this study was to determine the effect of parenteral recombinant human interferon (rHuIFN)-?2a administration on serum tumor necrosis factor (TNF)-?, interleukin (IL)-6 and IL-10 levels in healthy sheep. In addition, the main physiological parameters (rectal temperature, pulse rate, respiratory rate), hemogram and blood gas parameters were determined, as well. Materials and Methods: In this study, 9.000.000 IU rHuIFN-?2a was administered subcutaneously in 10 Merinos sheep. Blood samples were taken before (0 hours) and after at 4, 8, 12, 24, 48, 72, 96 and 120 hours. Rectal temperature, pulse and respiratory rate were also determined at the same sampling times. Sheep-specific TNF-?, IL-6 and IL-10 levels were determined from serum samples by the ELISA reader. Hemogram and blood gas parameters were measured by complete blood cell counter and blood gas analyzer, respectively. Results: After rHuIFN-?2a treatment, TNF-? and IL-10 concentrations reached peak levels (p<0.05) at the 96 hours, whereas the IL-6 level did not change in a statistically significant (p>0.05). On the other hand, a temporary increase in rectal temperature and pO2 levels (p<0.05) were determined, while decreased potassium and ionized calcium levels (p<0.05) were measured. Statistically significantly (p<0.05) fluctuations were determined in pulse rate, white blood cell counts, pH, base(ecf) and sodium values. Conclusion: It may be stated that administration of rHuIFN-?2a showed immunological effects in sheep, as it is generally accepted as safe and can be considered for use in treatment.
Açıklama
Anahtar Kelimeler
Koyun, interferon, sitokinler, hemogram, kan gazları, Sheep, cytokines, blood gases
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
4
Künye
Coşkun, D., İder, M., Arslan, M. S., Canbar, R., Yazar, E., (2020). The effect of interferon alpha administration on cytokine levels in sheep. Eurasian Journal of Veterinary Sciences, 36 (4), 324-330. DOI: 10.15312/EurasianJVetSci.2020.315
