Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem
Yükleniyor...
Dosyalar
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışma Konya’da abort problemli bir sığırcılık işletmesinde Bovine Viral Diarrhea Virus (BVDV) enfeksiyonunun varlığının belirlenmesi amacı ile yapıldı. Gereç ve Yöntem: İnfertilite ve abort problemi görülen 228 sığırdan kan serumu ve lökosit örnekleri toplanarak BVDV antijen ve BVDV’ye karşı gelişen antikorlar yönünden Enzyme Linked Immunosorbent Assay ile incelendi. Bulgular: Araştırmada 41 (%17.9) serum örneği seropozitif, 4 (%1.7) lökosit örneği BVDV antijen pozitif olarak belirlendi. BVDV antijen pozitif bulunan 4 sığırın 2 (%0.8)’si seropozitif 2 (%0.8)’si ise seronegatif tespit edildi. Antijen pozitif/antikor negatif hayvanlar 2 hafta sonra tekrar örneklendi. Seronegatif sığırlar için aynı sonuçlar elde edildi. Persiste enfekte oldukları belirlenen bu hayvanlar kesime gönderildi. Öneri: İşletmelere alınacak olan hayvanların kontrol edilerek hem BVDV antijen hem de antikor negatif olanların dahil edilmesi gerekmektedir.
Aim: The aim of this study is to determine the presence of Bovine Viral Diarrhea Virus (BVDV) infection in a cattle herd with abortion problem in Konya. Materials and Methods: Totally 228 blood serum and 228 leukocytes taken from cattle selected according to criteria for infertile and abortion problems were examined for antigens and antibodies to BVDV by Enzyme Linked Immunosorbent Assay. Results: In this research, 41 (17.9%) sera were found seropositive and 4 (1.7%) leukocytes were BVDV antigen positive. Of these 4 BVDV antigen positive cattle, a number of 2 (0.8%) were detected seropositive while 2 (0.8%) were seronegative. The animals being antigen positive and antibody negative were sampled second time after two weeks. The same results were detected for two seronegative cattle. The animals detecting persistent infection status were sent to slaughter. Conclusion: It is recommended that the animals should be checked in terms of BVDV for being negative both antigen and antibody before accepting them to the herds.
Aim: The aim of this study is to determine the presence of Bovine Viral Diarrhea Virus (BVDV) infection in a cattle herd with abortion problem in Konya. Materials and Methods: Totally 228 blood serum and 228 leukocytes taken from cattle selected according to criteria for infertile and abortion problems were examined for antigens and antibodies to BVDV by Enzyme Linked Immunosorbent Assay. Results: In this research, 41 (17.9%) sera were found seropositive and 4 (1.7%) leukocytes were BVDV antigen positive. Of these 4 BVDV antigen positive cattle, a number of 2 (0.8%) were detected seropositive while 2 (0.8%) were seronegative. The animals being antigen positive and antibody negative were sampled second time after two weeks. The same results were detected for two seronegative cattle. The animals detecting persistent infection status were sent to slaughter. Conclusion: It is recommended that the animals should be checked in terms of BVDV for being negative both antigen and antibody before accepting them to the herds.
Açıklama
Anahtar Kelimeler
Abort, BVDV, ELISA, sığır, Abortion, cattle
Kaynak
Eurasian Journal Of Veterınary Scıences
WoS Q Değeri
Scopus Q Değeri
Cilt
29
Sayı
3
Künye
Bulut, O., Avci, O., Yapici, O., Yavru, S., Simsek, A., (2013). Serological and virological investigation of Bovine Viral Diarrhea
Virus infection in cattle with abortion problem. Eurasian Journal of Veterinary Sciences, 29 (3), 159-162.
