Extraction of genomic DNA from polysaccharide- and phenolics-rich Ginkgo biloba

Basit Öğe Kaydı
dc.contributor.authorMaltas, Esra
dc.contributor.authorVural, Hasibe Cingilli
dc.contributor.authorYildiz, Salih
dc.date.accessioned2020-03-26T18:14:39Z
dc.date.available2020-03-26T18:14:39Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractOne prerequisite to reliable molecular biology work is that the genomic DNA of a sample should be of good quality. The isolation of intact, high-molecular-mass genomic DNA is essential for many molecular biology applications including long Polymerase Chain Reaction (PCR), endonuclease restriction digestion, Southern blot analysis, and genomic library construction. Many protocols are available for the extraction of DNA from plant material. DNA extraction of Ginkgo biloba is quite difficult to work on because of the high phenolic and polysaccharide content of its leaves. This study aimed to determine which protocol to use and which part of Ginkgo tree is most appropriate to extract good-quality genomic DNA. For this purpose, cetyltrimethylammonium bromide protocol and protocol of commercially available kit by EZ1 Nucleic acid isolation system have been optimized for extraction of genomic DNA from G. biloba leaves. Efficient yields of high-quality amplifiable DNA was produced rapidly with kit by EZ1 Nucleic acid isolation method. The purified DNA which has excellent spectral quality was efficiently amplified by 5 arbitrary primers (OPA11-15), and was suitable for long-fragment PCR amplification.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University (BAP)Selcuk University [08101019]en_US
dc.description.sponsorshipAuthors are very grateful to Scientific Research Foundation of Selcuk University (BAP) for providing foundation with 08101019 of project number. This study is a part of Esra Maltas's Ph.D thesis being continued.en_US
dc.identifier.endpage339en_US
dc.identifier.issn1996-0875en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage332en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26489
dc.identifier.volume5en_US
dc.identifier.wosWOS:000296009200005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherACADEMIC JOURNALSen_US
dc.relation.ispartofJOURNAL OF MEDICINAL PLANTS RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGenomic DNAen_US
dc.subjectrandom amplification of polymorphic DNA polymerase chain reactionen_US
dc.subjectDNA extractionen_US
dc.subjectphenolicsen_US
dc.titleExtraction of genomic DNA from polysaccharide- and phenolics-rich Ginkgo bilobaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu