Alcoholic extract of tarantula cubensis induces apoptosis in MCF-7 cell line
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Scientific Publishers of India
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Tarantula cubensis Alcoholic Extract (TCAE) is a homeopathic agent used for treating many disorders. This study aimed to define the effects of TCAE on the breast carcinoma cell line (MCF-7). After various concentrations (10, 20, 40, 80 and 160 µl/ml) of TCAE were applied to MCF-7 cells and the human embryonic kidney cell line (HEK293), the cells were incubated for 1, 3, 6, 9, 12, 24 and 48 h, followed by analysis by MTT assays. According to the results of the MTT assays, cells treated with 20 or 40 µl/ml TCAE for 6 h were applied to apoptosis analysis by flow cytometry. Secreted levels of tumor necrosis factor alpha (TNF?), interleukin (IL)-1?, IL-6, IL-10, Interferon-? (IFN?), Transforming Growth Factor-? (TGF?), and Nuclear Factor-kappa B (NF-?B) were measured using ELISAs. TNF? and TGF? levels increased while IL-6 and IL-10 levels fluctuated in MCF-7 cells. In conclusion, our study suggests that TCAE may change the normal cancer physiology and lead to cell death by activating apoptosis in MCF-7 cells. © 2017, Scientific Publishers of India. All rights reserved.
Açıklama
Anahtar Kelimeler
Apoptosis, Cytokines, MCF-7, Tarantula cubensis alcoholic extract, Theranekron
Kaynak
Biomedical Research (India)
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
28
Sayı
8
