Species Determination of Ancient Bone DNA From Fossil Skeletal Remains of Turkey Using Molecular Techniques

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dc.contributor.authorVural, Hasibe Cingilli
dc.contributor.authorTırpan, Ahmet Adil
dc.date.accessioned2020-03-26T18:05:02Z
dc.date.available2020-03-26T18:05:02Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe use of genetic technology in forensic science and archaeometry is applied primarily to distinguish between individials who may be the source of biological material associated with archeological remains. DNA sequences from ancient fossils have great potential for studies of phylogeny, biogeography and molecular evolution. DNA from fossils also facilitates the rigorous testing and calibration of mutation rates among related taxa, sex test and molecular divergence time (Cano et al., 1993; Burger et al., 1999). In this study, a rapid and quantitative ancient DNA extaction methods from human skeletal remains was developed for application of forensic science and archaeometry. For that reason, DNA was extracted from ancient human bones from Mugla in Turkey. Furthermore, all the bone samples which are obtained from burial place are subjected to DNA isolation and then interspecific sequence polymorphisms in the mitochondrial cytochrome b gene were analyzed by PCR to determine the species origin of Bronze Age animal and human skeletal remains. Existing techniques were refined by targeted primer design focusing on a DNA fragment shorter than 200 bp, an approach allowing us to identify up to all bone samples at the same time. For routine applications in archaeometry, food or material analyses, PCR may thus provide a simple alternative to sequencing of PCR products, allowing discrimination between species, even if the template DNA is degraded or contains traces of DNA from various species.en_US
dc.description.sponsorshipSelcuk UniversitySelcuk Universityen_US
dc.description.sponsorshipThe authors are grateful to Dr. A. Ahmet Tirpan and his working group for the access to valuable archaeological skeletal material. This study was partially supported by Selcuk University Archeometry Biotechnology Research Laboratory which belonged to Ass. Prof. Dr. Hasibe C. Vural and Scientific Research Foundation of Selcuk University (BAP) for providing foundation.en_US
dc.identifier.citationVural, H. C., Tırpan, A. A., (2010). Species Determination of Ancient Bone DNA From Fossil Skeletal Remains of Turkey Using Molecular Techniques. Scientific Research and Essays, 5(16), 2250-2256.
dc.identifier.endpage2256en_US
dc.identifier.issn1992-2248en_US
dc.identifier.issue16en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage2250en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/25276
dc.identifier.volume5en_US
dc.identifier.wosWOS:000282053800020en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorVural, Hasibe Cingilli
dc.institutionauthorTırpan, Ahmet Adil
dc.language.isoenen_US
dc.publisherACADEMIC JOURNALSen_US
dc.relation.ispartofScientific Research and Essaysen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAncient DNAen_US
dc.subjectSpecies Determinationen_US
dc.subjectCytochrome B Geneen_US
dc.subjectmtDNAen_US
dc.titleSpecies Determination of Ancient Bone DNA From Fossil Skeletal Remains of Turkey Using Molecular Techniquesen_US
dc.typeArticleen_US

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