Detection of Antibodies against Equine Herpes Virus-1 and Equine Herpes Virus-4 in Horses in Southeast Anatolia by Indirect Elisa
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
UNIV FED RIO GRANDE DO SUL
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Equine herpes viruses are a major cause of severe illness and mortality in domestic horses worldwide. Equine Herpes Virus-1 and Equine Herpes Virus-4 are genetically and antigenically related viruses. Equine Herpes Virus-1 infection is common in horses throughout the world, resulting in abortion and neonatal fetal death, respiratory disease, paresis, sporadic myelitis, myeloencephalopathy, and latent infections. Equine Herpes Virus-4, an important equine viral pathogen, causes respiratory tract disease in horses worldwide. The aim of this study was to investigate the presence of Equine Herpes Virus-1 and Equine Herpes Virus-4 antibodies in domestic horses in Southeast Anatolia. Materials, Methods & Results: In this study, the blood serum samples of 150 unvaccinated domestic horses were tested for equine herpes viruses including Equine Herpes Virus-1 and Equine Herpes Virus-4 specific antibodies. Blood serum samples were collected from the jugular vein of horses in five different provinces (Adiyaman, Diyarbakir, Gaziantep, Kilis, Sanliurfa) in Southeast Anatolia between November 2011 to January 2012. The presence of the Equine Herpes Virus-1 and Equine Herpes Virus-4 antibodies in the samples was determined with commercially available indirect Enzyme-Linked Immunosorbent Assay (ELISA) kits by using ELISA reader. The optical values of the micro plates were measured at 450 nm. The differences between Equine Herpes Virus-1 and Equine Herpes Virus-4 prevalence were evaluated with chi-square test (Minitab 14.0 Inc., State College, PA, USA). Difference were considered significant when P < 0.05. Equine Herpes Virus-1 and Equine Herpes Virus-4 specific antibodies were detected as in Adiyaman, Diyarbakir, Gaziantep, Kilis, Sanliurfa as 30% (9/30), 50% (15/30), 0% (0/30), 46.66% (14/30), 46.66% (14/30), 80%(24/30), 73.3% (22/30), 0% (0/30), 83.3% (25/30), 100% (30/30), respectively. Of the serum samples tested, 34.66% (52/150) and 67.33% (101/150) were found to be positive for Equine Herpes Virus-1 and Equine Herpes Virus-4 antibodies, respectively. Thirty horses were determined as seronegative both Equine Herpes Virus-1 and Equine Herpes Virus-4 infections in Gaziantep while 30 samples were found to be seropositive for Equine Herpes Virus-4 in Sanliurfa. Discussion: Equine Herpes Virus-1 and Equine Herpes Virus-4 belong to the Alphaherpesvirinae subfamily of the Herpesviridae family. Equine Herpes Virus-1 and Equine Herpes Virus-4 have DNA as their genetic material. Equine Herpes Virus-1 and Equine Herpes Virus-4 have a capsid, which displays icosahedral symmetry and is surrounded by a lipid envelope composed of various glycoproteins. Equine Herpes Virus-1 and Equine Herpes Virus-4 infections are common in equine animals in the worldwide. In epidemiological research on Alpha herpes viruses, the detection of Equine Herpes Virus-1 and Equine Herpes Virus-4 specific antibodies is used as an important indicator of the presence of symptomatic carriers in the population. Different laboratory tests can be used for the determination of specific antibodies for mentioned infections. ELISA is usually preferred in diagnosis of herpesviruses infections for its sensitivity and its economic advantages. Vaccination for Equine Herpes Virus-1 and Equine Herpes Virus-4 infections has not been applied in Turkey, so seropositivity results indicated that natural infections. In conclusion, Equine Herpes Virus-1 and Equine Herpes Virus-4 infections are widespread in horses in Southeast Anatolia, and protective measures, including vaccination, should be taken.
Açıklama
Anahtar Kelimeler
herpesviruses, equine herpes virus-1, equine herpes virus-4, ELISA
Kaynak
ACTA SCIENTIAE VETERINARIAE
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
42
