Investigation of genetic diversity and paternity in kangal white karaman rams using microsatellite markers

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dc.contributor.authorKurar E.
dc.contributor.authorBulut Z.
dc.contributor.authorÇağlayan T.
dc.contributor.authorGarip M.
dc.contributor.authorYilmaz A.
dc.contributor.authorNizamlioğlu M.
dc.date.accessioned2020-03-26T18:32:21Z
dc.date.available2020-03-26T18:32:21Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümüen_US
dc.description.abstractThe objectives of this study were to estimate genetic diversity and test possibility of conducting paternity testing at the DNA level as part of a breeding project of Kangal White Karaman sheep. As a pilot study, blood samples were collected from 13 rams that were used for breeding purposes in two different flocks in which level of inbreeding is proposed to be high. A total of 20 microsatellite markers were used to amplify genomic DNA by polymerase chain reaction (PCR). The resulting PCR products were separated by capillary electrophoresis and allele genotypes were determined. A total of 99 different alleles were determined ranging from 1 to 8 at each locus. The observed (Ho) and expected heterozygosities (He) values were ranged from 0.000 to 0.923 and from 0.000 to 0.871, respectively. Polymorphism information content (PIC) values were between 0.000 and 0.818. Total power of exclusion (PE) value was calculated as 0.999975 for twenty loci. Our results suggested that a panel, including the most informative twelve loci provides a total PE value of 0.999828, can be useful for parentage testing in Kangal White Karaman sheep.en_US
dc.identifier.citationKurar, E., Bulut, Z., Çağlayan, T., Garip, M., Yılmaz, A., Nizamlıoğlu, M. (2012). Investigation of Genetic Diversity and Paternity in Kangal White Karaman Rams Using Microsatellite Markers. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(6), 973-977.
dc.identifier.endpage977en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage973en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28677
dc.identifier.volume18en_US
dc.identifier.wosWOS:000311195800013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKurar E.
dc.institutionauthorBulut Z.
dc.institutionauthorÇağlayan T.
dc.institutionauthorGarip M.
dc.institutionauthorYilmaz A.
dc.institutionauthorNizamlioğlu M.
dc.language.isotren_US
dc.publisherVeteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGenetic diversityen_US
dc.subjectKangal white karaman sheepen_US
dc.subjectMicrosatelliteen_US
dc.subjectPaternity testingen_US
dc.titleInvestigation of genetic diversity and paternity in kangal white karaman rams using microsatellite markersen_US
dc.title.alternativeKangal akkaraman koçlarında genetik çeşitlilik ve ebeveyn testinin uygulanabilirliğinin mikrosatellit belirteçler kullanılarak araştırılmasıen_US
dc.typeArticleen_US

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