Phenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymer

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dc.contributor.authorYildiz, Huseyin Bekir
dc.contributor.authorCastillo, Jaime
dc.contributor.authorGuschin, Dmitrii A.
dc.contributor.authorToppare, Levent
dc.contributor.authorSchuhmann, Wolfgang
dc.date.accessioned2020-03-26T17:17:52Z
dc.date.available2020-03-26T17:17:52Z
dc.date.issued2007
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAn amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling. A two-layer sensor architecture was developed involving ascorbic acid oxidase entrapped within an electrodeposition polymer in a second layer on top of the redox polymer/tyrosinase layer. Using this sensor architecture it was possible to eliminate the current interference arising from direct ascorbate oxidation up to a concentration of 630 mu M ascorbic acid. The effects of the polymer thickness, the enzyme/polymer ratio, and the applied potential were evaluated with respect to optimal sensor properties. The sensitivity of the optimized sensors for catechol was 6.1 nA mu M-1 with a detection limit of 10 nM, and for phenol 0.15 nA mu M-1 with a detection limit of 100 nM.en_US
dc.identifier.doi10.1007/s00604-007-0768-1en_US
dc.identifier.endpage34en_US
dc.identifier.issn0026-3672en_US
dc.identifier.issn1436-5073en_US
dc.identifier.issue01.02.2020en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage27en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00604-007-0768-1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/21524
dc.identifier.volume159en_US
dc.identifier.wosWOS:000247472200003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGER WIENen_US
dc.relation.ispartofMICROCHIMICA ACTAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjecttyrosinaseen_US
dc.subjectphenolen_US
dc.subjectcatecholen_US
dc.subjectelectrodeposition painten_US
dc.subjectredox polymeren_US
dc.subjectsubstrate recyclingen_US
dc.subjectamperometric biosensoren_US
dc.subjectascorbic aciden_US
dc.subjectinterference eliminationen_US
dc.titlePhenol biosensor based on electrochemically controlled integration of tyrosinase in a redox polymeren_US
dc.typeArticleen_US

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