Polyvinylferrocenium based platinum electrodeposited amperometric biosensors for lysine detection

Basit Öğe Kaydı
dc.contributor.authorSahin, Ozlem Gokdogan
dc.contributor.authorGulce, Handan
dc.contributor.authorGulce, Ahmet
dc.date.accessioned2020-03-26T18:42:55Z
dc.date.available2020-03-26T18:42:55Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractTwo amperometric biosensors based on polyvinylferrocenium (PVF+) and platinum deposited PVF+ were constructed for lysine detection. The method for modification of electrodes relied on the electrooxidation of polyvinylferrocene in methylene chloride containing tetrabutylammonium perchlorate (TBAP) at +0.70 V and electrodeposition of platinum particles on the surface of PVF+ClO4- at -0.15 V vs. Ag/AgCl. Lysine oxidase (LyOx) enzyme was immobilized by ion exchange process into modified electrodes. The response of the resulted PVF(+)LyOx(-) and PVF(+)LyOx(-)/Pt biosensors determined by oxidation of enzymatically produced H2O2 at 0.60 V vs. Ag/AgCl. The influence of experimental conditions such as applied potential, pH, temperature on the biosensor responses were examined and analytical performance characteristics of the biosensors were determined. The experimental results of two biosensors compared with each other. The results showed that PVF(+)LyOx(-)/Pt biosensor had a linear range up to 3.0 mM whereas for PVF(+)LyOx(-) biosensor that range was linear up to 1.3 mM. The detection limits of PVF(+)LyOx(-)/Pt and PVF(+)LyOx(-) biosensors were determined to be about 6.5 x 10(-4) and 5.0 x 10(-4) mM, respectively. The response time of sensors was less than 30 s. The anti-interference ability and long-term stability of the biosensors were studied. The proposed biosensors were also used to determine lysine in pharmaceutical samples. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipSelcuk University Research Foundation (BAP)Selcuk University [08101018]en_US
dc.description.sponsorshipThe authors acknowledge financial support from the Selcuk University Research Foundation (BAP), Project number: 08101018.en_US
dc.identifier.doi10.1016/j.jelechem.2012.11.021en_US
dc.identifier.endpage7en_US
dc.identifier.issn1572-6657en_US
dc.identifier.issn1873-2569en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.jelechem.2012.11.021
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29734
dc.identifier.volume690en_US
dc.identifier.wosWOS:000317159000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE SAen_US
dc.relation.ispartofJOURNAL OF ELECTROANALYTICAL CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBiosensoren_US
dc.subjectLysine oxidaseen_US
dc.subjectLysineen_US
dc.subjectPlatinum nanoparticlesen_US
dc.titlePolyvinylferrocenium based platinum electrodeposited amperometric biosensors for lysine detectionen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu