The effect of oestradiol-17? on the motility, viability and the acrosomal status of bull sperm
Küçük Resim Yok
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
South African Bureau for Scientific Publications
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to test the effect of oestradiol-17? (E2) on the motility, viability and the acrosomal status of bull sperm in vitro. Pooled semen from Holstein bulls were incubated in the presence of 2, 4, and 8?g E2/mL for 24h. Semen was also incubated in media without E2. During the incubation, the number of motile, viable sperm and the number of sperm possessing lost/damaged acrosomes, cytoplasmic droplets and coiled tails were counted at 0, 4, 18 and 24h of incubation. Addition of 2?g E2/mL at 18h of incubation increased the total motility over the control. The number of forward progressing sperm was increased by the supplementation of 2 and 8?g E2/mL over the control group at the 4h incubation. Lower doses of E2 (2 and 4?g/mL) did not affect viability of sperm, but a high dose of E2 (8?g/mL) caused reductions in viability at 4 and 24h of incubation. The number of sperm cells with lost acrosomes was significantly high in control group at 24h of incubation. The number of sperm cells possessing proximal and distal cytoplasmic droplets and the number of sperm cells bearing coiled tails were not altered by any of the treatments. A small dose of E2 (2?g/mL) had a beneficial effect on the motility and acrosome integrity of bull sperm in vitro. Higher dose of E2 (8?g/mL) had a detrimental effect on viability. © South African Society for Animal Science.
Açıklama
Anahtar Kelimeler
Acrosome, Bovine males, Estradiol, In vitro incubation, Motility, Spermatozoa
Kaynak
South African Journal of Animal Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
40
Sayı
1
