Effect of oral immunoglobulin G in experimental necrotizing enterocolitis model [Deneysel nekrotizan enterokolit modelinde agizdan verilen immünglobülin G'nin etkisi]
Küçük Resim Yok
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Aim: Investigation of the protective effect of oral immunglobulin(Ig) G on rat intestinum in experimental necrotizing enterocolitis model. Materials and Methods: 40 newborn rats were devided into 4 groups each containing 10 rats. While control (C) group was fed by breast, the rats in necrotizing enterocolitis (N), sham (S), and treatment (T) groups were settled into incubators at 36°C and 60 % humidity and fed, but not by breast. The rats in group C were breast fed. The rats in N group were fed with Formula as soon as they were born. The rats in T group were fed with Formula and 1200 mg/kg/day oral Ig A with 4-hour intervals. The rats in S group were fed with Formula and 0.1 ml/kg/day distilled water which is solvent of Ig. The rats in all groups were weighed and sacrified on fourth day. 2 cm intestinal segment from proximal of ileocaecal valve was used for histopathologic examination, another 10 cm intestinal segment for biochemical examination. After laparotomy, H&E was used for histopathological examination and apoptosis repressor with card Ab-1 citt for immunohistochemical examination. Biochemical parameters such as TNF-?, and IL-6 were evaluated. Results: The rate of mortality in N, S, and T groups was significantly higher than groups C (P=0.038). Significant weight increase in group C was significantly higher than N, S, and T groups (P=0.000). Histopathologic parameters such as villus injury, transmural necrosis in group C were found to be significantly decreased compared with N, S, and T groups according to scoring system (P=0.000). Apoptosis in group C was found to be significantly decreased compared with N, S, and T groups according to scoring system (P=0.001). IL-6 and TNF-? levels were identified to be significantly decreased in group C compared with N, S, and T groups (P<0.05). There was no significant difference among N, S, and T groups (P>0.05). Conclusion: Pure IgG given orally was not identified to decrease intestinal damage and to prevent NEC in experimental NEC model both biochemically and histopathologically.
Açıklama
Anahtar Kelimeler
Immunoglobulin G, Necrotizing enterocolitis, Newborn, Treatment
Kaynak
Cocuk Cerrahisi Dergisi
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
22
Sayı
2
