In vitro antioxidant, cytotoxicity and chemical profile of different extracts from Acanthus hirsutus Boiss used in Anatolian folk medicine

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dc.contributor.authorUysal, Şengül
dc.contributor.authorAumeeruddy-Elalfi, Zaahira
dc.contributor.authorZengin, Gökhan
dc.contributor.authorAktümsek, Abdurrahman
dc.contributor.authorMaskovic, Pavle Z.
dc.contributor.authorVujic, Jelena M.
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.date.accessioned2020-03-26T19:54:19Z
dc.date.available2020-03-26T19:54:19Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroduction: The traditional use of phyto remedies and natural products may indicate their pharmacological potential. Screening plant extracts to identify their active components has gained momentum with possible beneficial effects for public health. The aim of study was to assess and compare the biological properties of different extracts (methanolic, aqueous, and ethyl acetate) prepared from Acanthus hirsutus (AH), a traditionally used medicinal plant. Methods: The phytochemical profile of AH was established via analysis of the total phenolic and flavonoid content, as well as the condensed tannins and gallotannins present in plants collected in the Anatolian region of Turkey. Chemical profiles were confirmed using the high-performance liquid chromatography (HPLC/DAD) method. Antioxidant potential were appraised through the analysis of their free radical scavenging activity, ferrous ion chelating ability, hydroxyl radical scavenging activity, total antioxidant capacity, and inhibition ability of lipid peroxidation. Any cytotoxicity activity of the extracts was assessed through the 3-[4,5-dimethylthiazol- 2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay against three different cancer cell lines. Results: The HPLC/DAD profile revealed the presence of rutin, p-hydroxybenzoic acid, vanillic acid, chlorogenic acid, and naringenin which were in significant abundance in the extracts. The aqueous extract had the highest antioxidant capacity with 284.33 +/- 0.05 mu g AAE/g extract, which correlated with the highest total condensed tannins, phenolics, and flavonoid contents (94.14 +/- 0.16 mg GAE/g extract, 114.30 +/- 0.11 mg GAE/g extract and 61.18 +/- 0.43 mg RE/g extract, respectively). Different sensitivities were observed towards the different cell lines used, with the aqueous extract being the less cytotoxic. Conclusion: AH proved to be a potent medicinal plant that can be further exploited as a complementary and alternative therapy for the management of oxidative stress related diseases such as cancer, diabetes, and rheumatoid arthritis.en_US
dc.identifier.doi10.1016/j.eujim.2017.12.009en_US
dc.identifier.endpage140en_US
dc.identifier.issn1876-3820en_US
dc.identifier.issn1876-3839en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage135en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.eujim.2017.12.009
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36706
dc.identifier.volume17en_US
dc.identifier.wosWOS:000427417700020en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.ispartofEUROPEAN JOURNAL OF INTEGRATIVE MEDICINEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAcanthus hirsutusen_US
dc.subjectCytotoxicityen_US
dc.subjectAntioxidanten_US
dc.subjectAnatoliaen_US
dc.subjectMedicinal plantsen_US
dc.subjectNatural productsen_US
dc.titleIn vitro antioxidant, cytotoxicity and chemical profile of different extracts from Acanthus hirsutus Boiss used in Anatolian folk medicineen_US
dc.typeArticleen_US

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