The in vivo investigation of apoptotic effects of Nigella sativa on carbon tetrachloride-induced hepatotoxicity

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dc.contributor.authorUysal, Tuna
dc.contributor.authorUgurtan, Mevlut Han
dc.contributor.authorSezer, Ela Nur Simsek
dc.contributor.authorVatansev, Husamettin
dc.contributor.authorBozkurt, Meryem
dc.contributor.authorEvliyaoglu, Nurcan
dc.date.accessioned2020-03-26T19:56:21Z
dc.date.available2020-03-26T19:56:21Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractNigella sativa commonly known as black seed or black cumin has been used for medicinal purposes for centuries. Our purpose in this study is to determine the apoptotic effects of black cumin seed oil (N. sativa) on CCl4 induced hepatoxicity via molecular markers. For this aim, four experimental groups were created and each of them was intraperitoneally exposed to CCl4 (G2), CCl4 + black cumin oil (G3) and black cumin oil (G4) except from control (G1). DNA fragmentation, RT-PCR, and caspase-3 assay were carried out. According to our results, a remarkable morphological anointment was observed at the G2. The G3 had the lower fatty comparing to G2. DNA profiles were visualized and DNA fragmentation was only in the G3. PCR results showed that BAX gene expression fairly rose in the G3 and BCL-2 gene expression was only determined in the G2. Decreased caspase-3 in G2, and the increased of it's in G3, clearly supported the activation of the apoptotic pathway for injured cells. Consequently, our results indicated that the increases of caspase and BAX/BCL-2 rates demonstrated that there was an effective molecular mechanism based on black cumin induced apoptosis in rat liver which leads to the release of cytochrome-c from the mitochondria to the cytosol, finally activating caspase-3 and resultant apoptosis.en_US
dc.description.sponsorshipBAP (Scientific Researching Projects) Foundation of Selcuk UniversitySelcuk University [13201052]en_US
dc.description.sponsorshipThe authors thanks, BAP (Scientific Researching Projects) Foundation of Selcuk University for financial support (Project number 13201052).en_US
dc.identifier.endpage250en_US
dc.identifier.issn0301-1208en_US
dc.identifier.issn0975-0959en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage245en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37050
dc.identifier.volume55en_US
dc.identifier.wosWOS:000444788400002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherNATL INST SCIENCE COMMUNICATION-NISCAIRen_US
dc.relation.ispartofINDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectApoptosisen_US
dc.subjectBlack cuminen_US
dc.subjectCaspase-3en_US
dc.subjectCCl4en_US
dc.subjectLiveren_US
dc.titleThe in vivo investigation of apoptotic effects of Nigella sativa on carbon tetrachloride-induced hepatotoxicityen_US
dc.typeArticleen_US

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