Controlled Trial of Efficacy of Dexketoprofen in Sciatic Nerve Crush Injury in Rats
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı, sıçanlarda siyatik sinir ezilme yaralanmalarında lokal olarak uygulanan deksketoprofen'in etkilerini karşılaştırmaktır. Bunun için sıçan modelinde yaralı siyatik sinirlerin Siyatik Fonksiyon Endeksi ( SFI ), Refleks Bacak geri çekilmesi (WRL), akson sayısı, parametreleri üzerinde 1 gün, 5 gün ve kontrol gruplarında akson çapı ve sinir çapı birlikte değerlendirilerek nöronal hasar incelendi. Yöntem ve Gereç: Wistar albino sıçanlar ( 21 hayvan ) kullanıldı. Sağ siyatik sinir bir Aesculap - Yaşargil anevrizma kullanılarak 10 saniye ezildi ve 10 saniye sonra anevrizma klibi açıldı. Deney hayvanları Grup 1, Grup 2 ve Grup 3 olarak üç gruba ayrıldı. Duyusal fonksiyonun iyileşmesi WRL ile değerlendirildi . Histo-patolojik inceleme yapıldı. Bulgular: Akson sayısı, akson çapı ve sinir çap bazında gruplar arasında anlamlı fark yoktu. Ama yaralanma indüksiyonundan sonra, istatistiksel olarak anlamlı fonksiyonel testler görüldü. Bu etki yalnızca çok dozlu Dex uygulamalarında etkili olarak bulundu. Sonuç: Bu çalışma; 5 gün üst üste deksketoprofen trometamol uygulanmasının, analjezik etkisi ile siyatik sinir hasarı üzerinde olumlu önemli etkilerinin olduğunu ortaya çıkarmıştır.
Object: Local applications of Dexketoprofen trometamol (dex) have been shown to increase in the sciatic nerve functional tests following the nerve injuries. The aim of the current study was to compare the efficacy of dex application for 1 and 5 days following nerve injury rat model. Possible effects of dex were checked by means of the Sciatic Function Index (SFI), Withdrawal of the Reflex Leg (WRL), number of axons, axon diameter, and nerve diameters. Material Methods: The animal crushed model was done through Aesculap -Yasargil aneurysm method on Wistar albino (N21) right sciatic nerves. In order to achieve desired purpose 3 experimental groups were organized: Group 1: control (injured and no drug); Group2: dexketoprofen 1 days (injured and single dose of drug); Group3: dexketoprofen 5 days (injured and single dose of 5 times drug applications). Injections were done through the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery of the sciatic nerves was evaluated with the improvement in the SFI values. Additionally, recovery of sensory function was assessed through WRL results and histopathological examination was performed 6 week following the injury. Results: At the end of the experimental periods there was no significant differences were found between the experimental group of animals on the bases of the number of axons, axon diameter, and nerve diameter. Moreover, up 30th day of the experimental period our functional test results have shown that there was no difference between the groups. But following the induction of the injury, the statistical significances were seen on the functional tests. This effect was seen only if the multi-dose application of the dex. Conclusions: This study suggests that; dexketoprofen trometamol of 5 consecutive days, has revealed positive significant changes in the sciatic nerve injury through analgesic effect of it.
Object: Local applications of Dexketoprofen trometamol (dex) have been shown to increase in the sciatic nerve functional tests following the nerve injuries. The aim of the current study was to compare the efficacy of dex application for 1 and 5 days following nerve injury rat model. Possible effects of dex were checked by means of the Sciatic Function Index (SFI), Withdrawal of the Reflex Leg (WRL), number of axons, axon diameter, and nerve diameters. Material Methods: The animal crushed model was done through Aesculap -Yasargil aneurysm method on Wistar albino (N21) right sciatic nerves. In order to achieve desired purpose 3 experimental groups were organized: Group 1: control (injured and no drug); Group2: dexketoprofen 1 days (injured and single dose of drug); Group3: dexketoprofen 5 days (injured and single dose of 5 times drug applications). Injections were done through the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery of the sciatic nerves was evaluated with the improvement in the SFI values. Additionally, recovery of sensory function was assessed through WRL results and histopathological examination was performed 6 week following the injury. Results: At the end of the experimental periods there was no significant differences were found between the experimental group of animals on the bases of the number of axons, axon diameter, and nerve diameter. Moreover, up 30th day of the experimental period our functional test results have shown that there was no difference between the groups. But following the induction of the injury, the statistical significances were seen on the functional tests. This effect was seen only if the multi-dose application of the dex. Conclusions: This study suggests that; dexketoprofen trometamol of 5 consecutive days, has revealed positive significant changes in the sciatic nerve injury through analgesic effect of it.
Açıklama
Anahtar Kelimeler
Nörolojik Bilimler
Kaynak
Journal of Neurological Sciences (Turkish)
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
31
Sayı
1
