Universal cell capture by immobilized antimicrobial peptide plantaricin

Basit Öğe Kaydı
dc.contributor.authorGuralp, Saadet Albayrak
dc.contributor.authorGubbuk, Ilkay Hilal
dc.contributor.authorKucukkolbasi, Semahat
dc.contributor.authorGulari, Erdogan
dc.date.accessioned2020-03-26T19:07:50Z
dc.date.available2020-03-26T19:07:50Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractPlantaricin-423 is a short antimicrobial peptide and displays bactericidal activities against several food-borne pathogens and spoilage Gram positive bacteria. The goal of this study was to investigate the potential of using immobilized plantaricin for capturing microorganisms on glass arrays. The peptide used for immobilization consists of N-terminal domain of Plantaricin-423 with an additional cysteine (Pln-17C) to form disulfide bonds with thiol groups present on silanized slides. Our results showed that Pln-17C is able to capture all six strains that were tested with varying affinities. The cell capture occurred within five minutes of incubation and the binding level was highest for Listeria innocua followed by other Gram positive strains tested. Pln-17C was also able to capture Escherichia coli with lower affinity, but the binding was significantly lower for Mycobacterium smegmatis compared to other strains. In addition, we have observed that immobilized Pln-17C maintained its anti-listerial activity; however, it did not kill E. coli as expected. Our results demonstrate the feasibility of utilizing antimicrobial peptides in biosensors for pathogen detection and for creating antimicrobial surfaces. Moreover, in combination with other peptides, different target species from food-borne pathogens to biodefense agents can be captured on more stable, economic, and robust platforms. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipGates FoundationGates Foundation; College of Engineeringen_US
dc.description.sponsorshipWe thank Dr. Meggie Grafton and Mina Zeinali in Professor Sunitha Nagrath's Lab for their assistance with fluorescence microscopy, Dr. Onnop Srivannavit for his help with slide preparation, and Professor Anish Tuteja for the use of the goniometer, all at The University of Michigan. Partial support of this study by a grant from the Gates Foundation and College of Engineering as well as an unrestricted gift from Biodiscovery-LLC is gratefully acknowledged.en_US
dc.identifier.doi10.1016/j.bej.2015.04.018en_US
dc.identifier.endpage22en_US
dc.identifier.issn1369-703Xen_US
dc.identifier.issn1873-295Xen_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage18en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.bej.2015.04.018
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32723
dc.identifier.volume101en_US
dc.identifier.wosWOS:000358969800003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofBIOCHEMICAL ENGINEERING JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBiomedicalen_US
dc.subjectBiosensorsen_US
dc.subjectImmobilizationen_US
dc.subjectAntimicrobial peptideen_US
dc.subjectCell captureen_US
dc.subjectViabilityen_US
dc.titleUniversal cell capture by immobilized antimicrobial peptide plantaricinen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu